中药金贯蓝提取物对寻常痤疮相关致病菌的体外抑菌作用  

In vitro antibacterial effect of Chinese herbal extracts of Jinguanlan on acne vulgaris-related pathogens

作　　者：万春梅 石春蕊[2] 廖蓓 魏玉辉[3] 高军[2] 张剑虹 WAN Chunmei;SHI Chunrui;LIAO Bei;WEI Yuhui;GAO Jun;ZHANG Jianhong(The First School of Clinical Medicine,Lanzhou University,Lanzhou,Gansu 730000,China;Department of Dermatology,The First Hospital of Lanzhou University,Lanzhou,Gansu 730000,China;Pharmacy Department,The First Hospital of Lanzhou University,Lanzhou,Gansu 730000,China)

机构地区：[1]兰州大学第一临床医学院,甘肃兰州730000 [2]兰州大学第一医院皮肤科,甘肃兰州730000 [3]兰州大学第一医院药剂科,甘肃兰州730000

出　　处：《安徽医药》2023年第7期1302-1307,共6页Anhui Medical and Pharmaceutical Journal

基　　金：甘肃省自然科学基金项目(20YF2FA009;22JR11RA043)。

摘　　要：目的考察自主研发的协定方中药“金贯蓝”提取物对寻常痤疮三种主要致病菌(痤疮丙酸杆菌、表皮葡萄球菌、金黄色葡萄球菌)的体外抑菌效果,为其临床治疗寻常痤疮提供一定实验依据。方法2021年1月至2022年3月采用琼脂打孔法考察金贯蓝提取物对三种痤疮相关致病菌的抑菌活性,并评估不同浓度药物对上述菌的抗菌作用是否具有浓度相关性;采用倍比稀释法测定药物最低抑菌浓度以及最小杀菌浓度,绘制药物时间抑菌曲线进一步考察其作用特点。结果500 g/L金贯蓝提取物对C.acnes、S.epidermidis、S.aureus的抑菌圈直径分别为(27.67±2.52)mm、(23.33±1.53)mm、(22.00±1.00)mm;浓度为250 g/L金贯蓝提取物对C.acnes、S.epidermidis、S.aureus的抑菌圈直径分别为(22.33±0.58)mm、(20.67±1.53)mm、(20.11±1.20)mm;浓度为100 g/L金贯蓝提取物对C.acnes、S.epidermidis、S.aureus的抑菌圈直径分别为(13.30±0.75)mm、(10.67±1.53)mm、(10.67±0.58)mm,抑菌圈直径与提取物浓度呈正相关关系(r>0.90,P<0.001)。金贯蓝提取物对痤疮丙酸杆菌、表皮葡萄球菌、金黄色葡萄球菌的最小抑菌浓度均为25 g/L;最小杀菌浓度分别为50 g/L、50 g/L、25 g/L。抑菌曲线表明其主要影响细菌生长的迟缓期。结论中药金贯蓝提取物对寻常痤疮三种主要致病菌(痤疮丙酸杆菌、表皮葡萄球菌和金黄色葡萄球菌)有较好的抑制作用,且与药物浓度呈正相关,值得进一步开发研究。Objective To investigate the effect of Jinguanlan extract on the three main pathogenic bacteria of acne vulgaris(Cutibacterium acnes,Staphylococcus epidermidis and Staphylococcus aureus),so as to provide a certain experimental basis for its clinical treatment of acne vulgaris.Methods From January 2021 to March 2022,Agar drilling method was used to investigate the antibacterial activity of the extract against three acne-related pathogenic bacteria,and to evaluate the concentration correlation of the antibacterial effect of different concentrations of drugs on the above-mentioned bacteria.The minimum inhibitory concentration and minimum bactericidal concentration were determined by multiple dilution method,and the time inhibition curve was drawn to further investigate its action characteristics.Results The bacteriostatic zone diameters of 500 g/L Jinguanlan extract against C.acnes,S.epidermidis and S.aureus were(27.67±2.52)mm,(23.33±1.53)mm and(22.00±1.00)mm,respectively.The bacteriostatic zone diameters of 250 g/L Jinguanlan extract against C.acnes,S.epidermidis and S.aureus were(22.33±0.58)mm,(20.67±1.53)mm and(20.11±1.20)mm,respectively.The bacteriostatic zone diameter of 100 g/L the extract against C.acnes,S.epidermidis and S.aureus were(13.30±0.75)mm,(10.67±1.53)mm and(10.67±0.58)mm,respectively.There was a positive correlation between the diameter of the antibacterial zone and the concentration of the extract(r>0.90 P<0.001).The MIC of Cutibacterium acnes,Staphylococcus epidermidis,and Staphylococcus aureus were all 25 g/L;MBC was 50 g/L,50 g/L,25 g/L,respectively.The bacteriostatic curve showed that it mainly affected the slow period of bacterial growth.Conclusion The extract of JINGUANLAN formula has a good inhibitory effect on the three main pathogenic bacteria of acne vulgaris,and it is positively correlated with the drug concentration,which is worthy of further development and research.

关 键 词：寻常痤疮 中草药 金贯蓝提取物 痤疮丙酸杆菌 表皮葡萄球菌 金黄色葡萄球菌 抑菌试验 

分 类 号：R285[医药卫生—中药学]