色氨酸对蛋种鸭抗氧化性能的影响  

Effect of Dietary Tryptophan Level on Antioxidant Ability in Laying Duck Breeders

作　　者：张亚男 黄雪冰 金永燕 赖跃 李凯潮 郑春田 ZHANG Yanan;HUANG Xuebing;JIN Yongyan;LAI Yue;LI Kaichao;ZHENG Chuntian(Institute of Animal Science,Guangdong Academy of Agricultural Sciences,Key Laboratory of Animal Nutrition and Feed Science(South China)of Ministry of Agriculture,State Key Laboratory of Livestock and Poultry Breeding,Guangdong Public Laboratory of Animal Breeding and Nutrition,Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition,Guangzhou Guangdong 510640)

机构地区：[1]广东省农业科学院动物科学研究所/畜禽育种国家重点实验室/农业农村部华南动物营养与饲料重点实验室/广东省畜禽育种与营养研究重点实验室,广东广州510640

出　　处：《广东畜牧兽医科技》2023年第3期35-41,共7页Guangdong Journal of Animal and Veterinary Science

基　　金：财政部和农业农村部⁃国家现代农业产业技术体系资助(CARS⁃42⁃13);广东省水禽产业技术创新团队(2019KJ137);广东省科协青年人才培育计划(SKXRC202213);广东省农业科学院高水平科技创新战略建设专项资金(202106TD,R2021PY⁃QY005);广东省畜禽疫病防治研究重点研究室开放课题(YDWS202106)。

摘　　要：该试验旨在通过研究饲粮色氨酸水平对蛋种鸭抗氧化性能的影响,探究蛋鸭饲粮色氨酸的适宜添加水平。试验选用体重和产蛋性能相近,健康的22周龄福建龙岩山麻蛋鸭504只,随机分为6个处理,每个处理6个重复,每个重复14只鸭,单笼饲养,试验期为16周。采用单因子完全随机设计,饲粮中色氨酸的水平分别为:1.0、2.0、3.0、4.0、5.0、6.0 g/kg。结果表明:1)饲粮色氨酸水平为2 g/kg时,蛋种鸭血浆T⁃SOD活性最高(P<0.05);血浆5⁃羟色胺活性随饲粮色氨酸水平升高呈线性和二次增加(P<0.05),对褪黑素无显著影响(P>0.05)。2)蛋种鸭肝脏T⁃SOD和T⁃AOC活性随饲粮色氨酸水平增加呈线性和二次增加,MDA含量在2 g/kg处最低,对GSH⁃Px活性无显著影响(P>0.05);肝脏GPx1、SOD和Nrf2表达量随饲粮色氨酸水平增加呈线性和二次变化,分别在4 g/kg、2 g/kg、1 g/kg处获得最大值(P<0.05)。3)蛋种鸭空肠T⁃SOD和T⁃AOC活性随饲粮色氨酸水平增加呈线性和二次增加,MDA在3 g/kg处含量最低(P<0.05),对GSH⁃Px含量无显著影响(P>0.05);HO⁃1和Nrf2表达量随饲粮色氨酸水平增加呈线性和二次变化增加(P<0.05),对SOD表达量无显著影响(P>0.05)。综上所述,饲粮中适宜色氨酸(2—3 g/kg),可通过增强蛋种鸭血浆、肝脏和空肠抗氧化酶活性,提高机体抗氧化性能。This study aimed to determine the effect of dietary tryptophan(Trp)levels on antioxidant ability and Trp requirement for laying duck breeders.A total of 504 Longyan breeding ducks(body weight:1.20±0.02 kg)aged 22 weeks were randomly allocated to 6 treatments,each with 6 replicates of 14 ducks.During the following 16 weeks,birds of different treatments were fed the basal diet with total Trp contents of 1.00,2.00,3.00,4.00,5.00 and 6.00 g/kg,respectively.The results showed that the dietary Trp level of 2 g/kg could result in the highest T⁃SOD activity in plasma(P<0.05),and the plasma serotonin content increased linearly and quadratically with dietary Trp supplemental levels(P<0.05).The content of T⁃SOD and T⁃AOC in liver increased linearly and quadratically,and hepatic MDA content was lowest with dietary Trp level being 2 g/kg(P<0.05).The gene expression of hepatic GPx1,SOD and Nrf2 increased linearly and quadratically,and the highest values was with Trp level of 4,2 and 1 g/kg,respectively(P<0.05).The content of T⁃SOD and T⁃AOC in jejunum increased linearly and quadratically,and jejunal MDA content was lowest with the dietary Trp level of 3 g/kg(P<0.05).The gene expression of jejunal HO⁃1 and Nrf2 increased linearly and quadratically(P<0.05).Collectively,dietary Trp level of 2 to 3 g/kg could improve the antioxidant ability in plasma,liver and jejunum in laying breeding ducks.

关 键 词：色氨酸 蛋种鸭 抗氧化性能 

分 类 号：S816.71[农业科学—饲料科学]