一例母猪混合感染猪圆环病毒3型与近平滑假丝酵母菌的病例报告  

A Case Report on Co􀆼infection of Porcine Circovirus Type 3 and Candida parapsilosis in Sows

作　　者：薛少华 吕紫欣 张鑫杰 魏梅蕊 黄紫芯 林新龙 黄思琼 华贤通 戴爱玲[1,2,3] 杨小燕 俞国华[1,2,3] XUE Shaohua;LÜZixin;ZHANG Xinjie;WEI Meirui;HUANG Zixin;LIN Xinlong;HUANG Siqiong;HUA Xiantong;DAI Ailing;YANG Xiaoyan;YU Guohua(College of Life Sciences,Longyan University,Longyan Fujian 364012;Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology,School of Life Sciences,Longyan University,Longyan Fujian 364012;Fujian Engineering Research Center for Swine Disease Control and Prevention,Longyan 364012;Longyan Xinmu Biotechnology Co.,Ltd,Longyan Fujian 364000;Fujian Best Agricultural Development Co.,Ltd,Longyan Fujian 364000)

机构地区：[1]龙岩学院生命学科学院,福建龙岩364012 [2]福建省家畜传染病防治与生物技术重点实验室,福建龙岩364012 [3]福建省生猪疫病防控工程技术研究中心,福建龙岩364000 [4]龙岩欣牧生物科技有限公司,福建龙岩364000 [5]福建佰思拓农业发展有限公司,福建龙岩364000

出　　处：《广东畜牧兽医科技》2023年第3期80-84,共5页Guangdong Journal of Animal and Veterinary Science

基　　金：福建省自然科学基金(2019J01801);福建省科技重大专项(2019NZ09005)。

摘　　要：2022年6月福建省龙岩市某规模化猪场的母猪陆续发病,临床主要表现为病猪的膝关节轻微肿大,不愿久站,甚至站立困难。为查明发病原因,对病猪进行病理剖检,并采集血液和膝关节积液样品,分别用于生猪常见疫病病原体的核酸检测和微生物的分离培养。结果显示:病理剖检未发现主要脏器的明显病变;血液样品的核酸检测显示,猪圆环病毒3型(Por⁃cine Circovirus type 3,PCV3)为阳性,未检出其它主要病原体;膝关节积液样品的微生物培养产物的显微镜镜检结果显示有酵母样真菌,经培养产物的PCR扩增和DNA测序表明:该酵母样真菌为近平滑假丝酵母菌(Candida parapsilosis)。结论:该病例为猪圆环病毒3型(PCV-3)与近平滑假丝酵母菌的混合感染。根据文献查询,这是世界首例有关近平滑假丝酵母菌导致生猪膝关节深部感染并从膝关节积液中成功分离到近平滑假丝酵母菌的报道。In June 2022,sows in a large􀆼scale pig farm in Longyan City,Fujian Province were reported ill in succession.The main clinical symptoms include slight swollen knee joints,reluctant to move or even difficulty in standing.In order to find out the causes of the disease,pathological examination was carried out on the diseased pigs.Blood samples were collected for nucleic acid detection of common pathogens and knee joint synovial fluid samples were collected for the isolation of microorganisms.The results showed that no obvious pathological changes were found in the main organs.The nucleic acid detection results of blood samples showed that porcine circovirus type 3(PCV3)was positive and no other major pathogens were detected.Microscopic examination of the microbial culture product of knee joint synovial fluid sample showed that there was yeast⁃like fungus.PCR amplification and DNA sequencing of the cultured products showed that the yeast⁃like fungus was Candida parapsilosis.Conclusion:This case is a mixed infection of PCV⁃3 and Candida parapsilosis.According to the literature,this is the first case in the world to report that Candida parapsilosis caused deep knee joint infection in pigs and Candida parapsilosis was successfully isolated from knee joint synovial fluid.

关 键 词：母猪 猪圆环病毒3型 近平滑假丝酵母菌 混合感染 

分 类 号：S858.28[农业科学—临床兽医学]