Genome-wide profiling of histone H3 lysine 27 trimethylation and its modification in response to chilling stress in grapevine leaves  

作　　者：Zhenfei Zhu Qingyun Li Duncan Kiragu Gichuki Yujun Hou Yuanshuang Liu Huimin Zhou Chen Xu Linchuan Fang Linzhong Gong Beibei Zheng Wei Duan Peige Fan Qingfeng Wang Haiping Xin 

机构地区：[1]Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture,Core Botanical Gardens/Wuhan Botanical Garden,Chinese Academy of Sciences,Wuhan,Hubei,430074,China [2]Sino-Africa Joint Research Center,Chinese Academy of Sciences,Wuhan,Hubei,430074,China [3]Key Laboratory of Plant Resources,Institute of Botany,Chinese Academy of Science,Beijing,100093,China [4]Institute of Fruit and Tea,Hubei Academy of Agricultural Sciences,Wuhan,Hubei,430064,China [5]University of Chinese Academy of Sciences,Beijing,100049,China [6]Wuhan Academy of Agricultural Sciences,Wuhan,Hubei,430074,China

出　　处：《Horticultural Plant Journal》2023年第3期496-508,共13页园艺学报（英文版）

基　　金：supported by the National Key Research and Development Program of China (Grant No. 2018YFD1000300);the National Natural Science Foundation of China (Grant No. 32025032);the Grape Breeding Project of Ningxia (Grant No. NXNYYZ202101-04);Major Program of Technological Innovation in Hubei Province (Grant No. 2019ABA093).

摘　　要：Histone H3 lysine 27 trimethylation(H3K27me3) is a histone modification associated with transcriptional repression. However, insights into the genome-wide pattern of H3K27me3 in grapevines are limited. Here, anti-H3K27 chromatin immunoprecipitation(ChIP), high-throughput sequencing, and transcriptome analysis were performed using leaves of Vitis amurensis. The leaves were treated at 4°C for 2 h and 24 h and used to investigate changes in H3K27me3 under chilling treatment. The results show that H3K27me3 is well-distributed both in gene regions(-50%) and in the intergenic region(-50%) in the grapevine genome(Vitis vinifera ‘Pinot Noir PN40024'). H3K27me3 was found to be localized in8 368 annotated gene regions in all detected samples(leaves at normal temperature and under chilling treatments) and mainly enriched in gene bodies with the adjacent promoter and downstream areas. The short-term chilling treatments(4°C for 2 h) induced 2 793 gains and 305losses in H3K27me3 modification. Subsequently, 97.3% of the alterations were restored to original levels after 24 h treatment. The ChIP-qPCR for five differential peaks showed similar results to the data for ChIP-seq, indicating that the chilling-induced H3K27me3 modification is reliable.Integrative analysis of transcriptome and ChIP-seq results showed that the expression of H3K27me3 target genes was significantly lower than those of non-target genes, indicating transcriptional repression of H3K27me3 in grapevine leaves. Furthermore, histone methylation alterations were detected in 82 genes and were related to either repression or activation of their expression during chilling stress. The findings provide the genome-wide H3K27me3 patterns in grapevines and shed light on uncovering its regulation in chilling stress responses.

关 键 词：Vitis amurensis Histone modification H3K27me3 Chilling stress 

分 类 号：S663.1[农业科学—果树学]