Epigenetic modification mechanisms of chloroplasts mutants in pineapple leaves during somatic regeneration  

作　　者：Yanhui Liu S.V.G.N.Priyadarshani Meirong Chi Maokai Yan Mohammad Aqa Mohammadi Man Zhang Qiao Zhou Lulu Wang Tiantian Luo Myat Hnin Wai Xiaomei Wang Hanyang Cai Haifeng Wang Yuan Qin 

机构地区：[1]College of Life Sciences,Key Lab of Genetics,Breeding and Multiple Utilization of Crops,Ministry of Education,Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology,Center for Genomics and Biotechnology,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China [2]College of Life Science,Longyan University,Longyan,Fujian 364012,China [3]Guangxi Key Laboratory of Sugarcane Biology,State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,College of Agriculture,Guangxi University,Nanning,Guangxi 530004,China [4]Horticulture Research Institute,Guangxi Academy of Agricultural Sciences,Nanning Investigation Station of South Subtropical Fruit Trees,Ministry of Agriculture,Nanning,Guangxi 530007,China [5]Pingtan Science and Technology Research Institute of Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China [6]National Education Commission,Sri Lanka

出　　处：《Horticultural Plant Journal》2023年第3期509-522,共14页园艺学报（英文版）

基　　金：supported by the Science and Technology Major Project of Guangxi (Grant No. Guike AA22068096);Guangxi Distinguished Experts Fellowship to YQ, Science and Technology Major Project of Guangxi (Grant No. Guike 2018-266-Z01);Project of Guangxi featured fruit innovation team on pineapple breeding and cultivation post under national modern agricultural industry technology system (Grant No. nycytxgxcxtd-17-05);Science and Technology Program of Fujian Province (Grant No. 2019N5008);Science and technology innovation project of Pingtan Science and Technology Research Institute (Grant Nos. PT2021007, PT2021003);China Postdoctoral Science Foundation (Grant No. 2018M632564);the National Natural Science Foundation of China (31970333, U1605212)。

摘　　要：Somaclonal variation in tissue culture is a common phenomenon induced by various external or internal environmental conditions,resulting in heritable or non-heritable alterations in gene expression. One crucial mechanism involved in plant growth and development is epigenetic regulation. A highly dynamic epigenome can respond to environmental changes by regulating gene expression. DNA methylation is one of these epigenetic modifications that can alter gene expression in tissue-cultured pineapple plants. The underlying mechanism of such somaclonal variations in pineapple and the epigenetic regulation involvement in somaclonal variations has not been studied. This study performed DNA methylome and transcriptome sequencing of wild-type(WT) and mutant pineapple plants(WS, HW, and TW). We observed altered DNA methylation patterns in chlorophyll development in the mutants. Specifically, we noticed that the methylation levels in the CHG and CHH contexts were lower in the gene body regions compared to the upstream and downstream regions. We identified several thousand differentially methylated regions(DMRs) located at the gene body regions, some of which overlapped with the differentially expressed genes(DEGs). Functional enrichment analyses suggested that these genes were involved in chlorophyll metabolism. Thus, our results revealed that the transcriptional regulation of many chlorophyll metabolic essential genes could be regulated by DNA methylation caused by somaclonal variations and provided insights into epigenetic mechanisms underlying the regulation of chlorosis in pineapple plants.

关 键 词：PINEAPPLE DNA methylation TRANSCRIPTOME Chlorophyll metabolism 

分 类 号：S668.3[农业科学—果树学]