Epigenetic changes in the regulation of carotenoid metabolism during honeysuckle flower development  

作　　者：Hang Yu Na Cui Kun Guo Wen Xu Haifeng Wang 

机构地区：[1]State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,Guangxi Key Lab of Sugarcane Biology,College of Agriculture,Guangxi University,Nanning,Guangxi 530005,China [2]Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Peking Union Medical College,Beijing 100193,China [3]Innovation and Transformation Center of Science and Technology,College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350122,China

出　　处：《Horticultural Plant Journal》2023年第3期577-588,共12页园艺学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Grant Nos. 32160142, 81873095)。

摘　　要：Flower development is one of the most vital pathways in plant development, during which the epigenetic regulation of gene expression is essential. DNA methylation, the most conserved epigenetic modification, participates in gene expression regulation and transposable element silencing. Honeysuckle(Lonicera japonica) is an important medicinal plant renowned for its colorful and fragrant flowers. Honeysuckle flowers change color from white to gold as a result of carotenoid accumulation during development. However, the role of DNA methylation in flower color changes is not well understood in L. japonica. Here, we performed whole-genome bisulfite sequencing and transcriptome sequencing during flowering development in honeysuckle. The results showed that a decrease in the levels of genome-wide average DNA methylation during flower development and changes in DNA methylation were associated with the expression of demethylase genes. Moreover, many genes involved in carotenoid biosynthesis and degradation, such as Lj PSY1, LjPDS1, LjLCYE, and LjCCD4, have altered expression levels because of hypomethylation, indicating that DNA methylation plays an important role in flower color changes in honeysuckle. Taken together, our data provide epigenetic insights into flower development and color change in honeysuckles.

关 键 词：Lonicera japonica DNA methylation Epigenetic regulation Gene expression Carotenoid metabolism 

分 类 号：S567.79[农业科学—中草药栽培]