马占相思开放式组培体系的建立  

Establishment of open tissue culture system for Acacia mangium

作　　者：卢艳平 陈玉军[1] 李玫[1] 黄烈健[1] LU Yanping;CHEN Yujun;LI Mei;HUANG Liejian(Research Institute of Tropical Forestry,Chinese Academy of Forestry,Guangzhou,Guangdong 510520,China;College of Forestry,Nanjing Forestry University,Nanjing,Jiangsu 210037,China)

机构地区：[1]中国林业科学研究院热带林业研究所,广东广州510520 [2]南京林业大学林学院,江苏南京210037

出　　处：《西北农林科技大学学报（自然科学版）》2023年第7期107-114,共8页Journal of Northwest A&F University(Natural Science Edition)

基　　金：中央公益性科研院所基本科研业务费专项基金(CAFYBB2017MB009)。

摘　　要：【目的】研究基本培养基和不同质量浓度植物生长调节剂在开放组培条件下对马占相思(Acacia mangium)芽诱导、增殖和生根的影响,为建立马占相思开放式组培体系提供技术支持。【方法】以马占相思含饱满腋芽的带叶茎段作为外植体,研究MS、1/2 MS、1/4 MS和1/8 MS培养基加入不同质量浓度6-BA(0.5,1.0,1.5 mg/L)对马占相思芽诱导的影响;采用L_(9)(3^(4))正交试验设计,探讨不同质量浓度6-BA(1.5,2.0,2.5 mg/L)、IBA(0.1,0.5,1.0 mg/L)和IAA(0.10,0.25,0.50 mg/L)组合对马占相思芽增殖的影响;同时,研究不同质量浓度IBA(0.5,1.0,1.5,2.0 mg/L)和NAA(0.1,0.5,1.0 mg/L)组合对马占相思组培苗生根的影响。【结果】马占相思最佳芽诱导培养基为1/8 MS+0.5 mg/L 6-BA+0.2 g/L百菌清,芽诱导率为91.87%;影响马占相思增殖的生长调节剂主次顺序为6-BA>IAA>IBA,最佳增殖培养基为MS+2.0 mg/L 6-BA+0.1 mg/L IBA+0.25 mg/L IAA+0.2 g/L百菌清,增殖倍数为3.38;马占相思最佳生根培养基为2.0 mg/L IBA+1.0 mg/L NAA+0.2 g/L百菌清,生根率为80.00%。【结论】初步建立了马占相思开放式组培技术体系,为马占相思无性系产业化扩繁提供了一种新方法。【Objective】This study investigated the effects of basic medium and plant growth regulators at different concentrations on induction,proliferation and rooting of Acacia mangium buds under open tissue culture conditions to provide support for the establishment of open tissue culture system.【Method】Semi-lignified annual shoots with axillary buds of A.mangium were used as explants to study the effects of culture medium(MS,1/2 MS,1/4 MS and 1/8 MS)and 6-BA addition(0.5,1.0 and 1.5 mg/L)on buds induction of A.mangium.The L_(9)(3^(4))orthogonal experimental design was used to investigate the effects of 6-BA(1.5,2.0 and 2.5 mg/L),IBA(0.1,0.5 and 1.0 mg/L)and IAA(0.10,0.25 and 0.50 mg/L)on proliferation of axillary buds of A.mangium.The effects of IBA(0.5,1.0,1.5 and 2.0 mg/L)and NAA(0.1,0.5 and 1.0 mg/L)on rooting of A.mangium tissue culture seedlings were also studied.【Result】The best bud induction medium of A.mangium was 1/8 MS+0.5 mg/L 6-BA+0.2 g/L chlorothalonil,and the bud induction rate was 91.87%.The effects of growth regulators for proliferation were in the order of 6-BA>IAA>IBA.The optimal proliferation medium was MS+2.0 mg/L 6-BA+0.1 mg/L IBA+0.25 mg/L IAA+0.2 g/L chlorothalonil,and the multiplication factor was 3.38.The best rooting medium for A.mangium was 2.0 mg/L IBA+1.0 mg/L NAA+0.2 g/L chlorothalonil,and the obtained rooting rate was 80.00%.【Conclusion】The open tissue culture system of A.mangium was preliminarily established,providing a new method for the industrialization and propagation of A.mangium clones.

关 键 词：马占相思 开放式组织培养 芽诱导 无性繁殖 

分 类 号：S792.99[农业科学—林木遗传育种]