生防菌长枝木霉菌株T6及其代谢产物对立枯丝核菌拮抗作用  

Antagonism of Trichoderma longibrachiatum Isolate T6 and Its Metabolites to Rhizoctonia solani

作　　者：李昕冉 景涛 孙晨曦 郭彩苹 马耀杰 徐秉良 张树武 LI Xinran;JING Tao;SUN Chenxi;GUO Caiping;MA Yaojie;XU Bingliang;ZHANG Shuwu(College of Plant Protection,Gansu Agricultural University/Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学植物保护学院/甘肃省农作物病虫害生物防治工程实验室,兰州730070

出　　处：《西北农业学报》2023年第7期1131-1137,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：甘肃农业大学“伏羲杰出人才培育计划”项目(Gaufx-03J03);甘肃省杰出青年基金(18JR3RA161);甘肃农业大学人才专项经费(2017RCZX-07)。

摘　　要：为了开发对立枯丝核菌(Rhizoctonia solani)具有较好拮抗作用的生防制剂,采用对峙培养法、含药培养基法和显微观察法,测定生防菌长枝木霉(Trichoderma longibrachiatum)菌株T6及其代谢产物对立枯丝核菌的抑制作用活性,初步探究长枝木霉菌株T6的作用机制。结果表明,长枝木霉菌株T6对立枯丝核菌生长具有显著的抑制作用,对峙培养5 d时菌株T6不仅对立枯丝核菌营养生长抑制率为66.50%,而且对其菌丝具有明显重寄生作用。菌株T6发酵液及其代谢产物对立枯丝核菌营养生长也具有显著的抑制效果,培养4 d时其对立枯丝核菌的生长抑制率分别为72.29%和71.91%。因此,长枝木霉菌株T6对立枯丝核菌具有显著的拮抗作用,主要通过对病原菌的重寄生作用及其代谢产物发挥拮抗作用。To develop an antifungal biocontrol agent to Rhizoctonia solani,and to preliminarily explore mechanism of antifungal activity of the isolate T6,the combined methods of confrontation culture,drug-containing medium and microscopic observation were used to determine the antifungal activity of Trichoderma longibrachiatum isolate T6 and its metabolites against Rhizoctonia solani.The results showed that the isolate T6 had a significant inhibition effect on the vegetative growth of R.solani,the inhibition rate was 66.50%at 5 days after culture,and also the hyperparasitism of the isolate T6 to R.solani was observed.The fermentation broth and metabolites of the isolate T6 represented an inhibition rate of 72.29%and 71.91%against R.solaniat 4 days after culture,respectively,there was a remarkable inhibition effect to R.solani.As a result,the isolate T6 possesses an obvious antagonism by hyperparasitism of the isolate T6 and its metabolites.

关 键 词：生防 木霉 代谢产物 拮抗作用 生长抑制率 

分 类 号：S476[农业科学—农业昆虫与害虫防治]