中药抑制间充质干细胞衰老的作用及机制  

Effect and mechanism of traditional Chinese medicine on inhibiting the aging of mesenchymal stem cells

作　　者：潘小龙 樊飞燕 应春苗 刘飞祥 张运克[1,2] Pan Xiaolong;Fan Feiyan;Ying Chunmiao;Liu Feixiang;Zhang Yunke(Henan University of Chinese Medicine,Zhengzhou 450046,Henan Province,China;First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450046,Henan Province,China)

机构地区：[1]河南中医药大学,河南省郑州市450046 [2]河南中医药大学第一附属医院,河南省郑州市450046

出　　处：《中国组织工程研究》2024年第7期1091-1098,共8页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81974564),项目负责人:张运克;国家自然科学基金(82104730),项目负责人:刘飞祥;中原英才计划—科技创新领军人才项目(224200510027),项目负责人:张运克。

摘　　要：背景:间充质干细胞的衰老严重影响其临床应用并且是衰老相关疾病发生的主要诱因之一,中药具有良好的抗衰老作用,可抑制间充质干细胞衰老从而促进其在组织工程中的应用并防治衰老相关疾病。目的:综述中药抑制间充质干细胞衰老的作用及机制。方法:检索中国知网和PubMed数据库2012-2022年关于中药抑制间充质干细胞衰老的文献,以“中药,间充质干细胞,衰老”为中文检索词,以“traditional chinese medicines,mesenchymal stem cells(MSCs),aging”为英文检索词,最终共纳入92篇文献进行分析。结果与结论:(1)文章总结了当前间充质干细胞衰老的5个主要机制:包括DNA损伤、端粒缩短、氧化应激、自噬障碍及线粒体功能障碍。(2)文章梳理了衰老间充质干细胞的表型特征,主要包括细胞体积增大、增殖和多向分化能力下降、β-半乳糖苷酶活性增加、p21及p16通路激活等。(3)文章总结了当前中药抑制间充质干细胞衰老的主要机制,包括抑制Wnt通路激活、抑制线粒体活性氧产生、促进沉默信息调节因子2同系物1、磷脂酰肌醇3-激酶/蛋白激酶B、腺苷酸活化蛋白激酶及核因子E2相关因子2通路激活、促进端粒酶反转录酶表达等。(4)目前在中药抑制间充质干细胞衰老的研究中以对骨髓间充质干细胞研究最多,效果较好。(5)左归丸、补肾调肝方、白藜芦醇及黄芪等中药均可通过促进衰老间充质干细胞增殖及成骨分化起到防治骨质疏松作用,但对中药通过抑制间充质干细胞衰老改善间充质干细胞旁分泌功能及防治其他衰老相关疾病的机制仍需进一步探究。BACKGROUND:The aging of mesenchymal stem cells is one of the main causes of aging-related diseases,and seriously affects its clinical application.Traditional Chinese medicine has a good anti-aging effect,and it can inhibit the aging of mesenchymal stem cells to promote its application in tissue engineering and prevent and treat aging-related diseases.OBJECTIVE:To review the effect and mechanism of traditional Chinese medicine on inhibiting the aging of mesenchymal stem cells.METHODS:We searched CNKI and PubMed for the literature on inhibiting mesenchymal stem cell aging with traditional Chinese medicine from 2012 to 2022.The keywords were“traditional Chinese medicine,mesenchymal stem cells(MSCs),aging”in Chinese and English,respectively.Finally,92 articles were included for further review.RESULTS AND CONCLUSION:(1)We summarized five main mechanisms of the aging of mesenchymal stem cells:DNA damage,telomere shortening,oxidative stress,autophagy disorder and mitochondrial dysfunction.(2)This paper reviewed the phenotypic characteristics of senescent mesenchymal stem cells,including increases in cell volume,decreases in proliferation and multi-directional differentiation,increases inβ-galactosidase activity,and activation of p21 and p16 pathways,and so on.(3)We summarized the main mechanisms of Chinese medicine inhibiting the senescence of mesenchymal stem cells at present,including inhibiting the activation of the Wnt pathway,inhibiting the production of mitochondrial reactive oxygen species,promoting the silencing of information regulator factor 2 homolog 1,phosphatidylinositol 3-kinase/protein kinase B,adenosine 5’-monophosphate-activated protein kinase and nuclear factor E2 related factor 2 pathway activation,and promoting the expression of telomerase reverse transcriptase.(4)At present,bone marrow mesenchymal stem cells are the most widely studied in the research of traditional Chinese medicine to inhibit the aging of bone marrow mesenchymal stem cells,and the effect is better.(5)Zuogui Wan,Bushen Tiaoga

关 键 词：中药 间充质干细胞 衰老 端粒 氧化应激 自噬 线粒体 

分 类 号：R453[医药卫生—治疗学] R363[医药卫生—临床医学] R285