基于网络药理学的苗药土知母治疗肝硬化的药理机制研究  

Study on the pharmacological mechanism of the treatment of cirrhosis with Rhizoma Anemarrhenae of Miao medicine based on network pharmacology

作　　者：张宁 李玲 叶涛 ZHANG Ning;LI Ling;YE Tao(The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang,Guizhou 550001,China)

机构地区：[1]贵州中医药大学第一附属医院,贵州贵阳550001

出　　处：《现代医药卫生》2023年第11期1822-1828,1833,共8页Journal of Modern Medicine & Health

基　　金：贵州省“十四五”中医药、民族医药重点学科-临床中药学[QZYYZDXK(JS)-2021-03];贵阳中医学院第一附属医院博士启动基金(GYZYYFY-BS-2019(03))。

摘　　要：目的构建苗药土知母活性成分-作用靶点-肝硬化疾病-通路网络图,对靶点涉及的功能和通路进行分析,探讨苗药土知母治疗肝硬化的作用机制。方法通过文献挖掘、TCMSP、TCM@Taiwan和TCMID数据库检索苗药土知母主要活性成分,利用Genecards、OMIM、TTD和MalaCards数据库预测和筛选肝硬化的作用靶点,两者取交集得到苗药土知母治疗肝硬化的靶点。采用Cytoscape3.7.1软件构建苗药土知母活性成分-靶点-肝硬化-通路网络互作图。采用ClueGo数据库对靶点进行GO富集分析和KEGG通路分析。结果获得苗药土知母23个活性成分,涉及383个靶点。肝硬化涉及3410个基因,两者交集基因有103个。苗药土知母可能通过介导肝硬化中肿瘤坏死因子相关凋亡诱导配体激活的凋亡信号通路的正调控、铁对翻译起始的正调控和丝裂原活化蛋白激酶K活性失活的靶点,及其参与的肝硬化中的缺氧诱导因子1信号通路、催乳素信号通路和叉形头转录因子O信号通路等25条通路治疗肝硬化(P≤0.01)。结论网络药理学可用于初步预测苗药土知母治疗肝硬化的潜在靶点,这为进一步的药理机制研究提供科学依据。Objective To construct the network diagram of active constituents-target sites-cirrhosis disease-pathway of Rhizoma Anemarrhenae of Miao medicine,and analyze the functions and pathways involved in the target sites,so as to explore the mechanism of the treatment of cirrhosis with Rhizoma Anemarrhenae of Miao medicine.Methods The main active ingredients of Rhizoma Anemarrhenae of Miao medicine were obtained by TCMSP,TCM@Taiwan,and TCMID.The target of liver cirrhosis was predicted and screened by Genecards,OMIM,TTD and MalaCards databases.The target of liver cirrhosis was obtained by intersection of the two databases.Cytoscape 3.7.1 software was used to construct the network interaction map of active constituents-target sites-cirrhosis-pathway.ClueGo database was used for GO enrichment analysis and KEGG pathway analysis.Results The results showed that 23 active components and 383 targets of Rhizoma Anemarrhenae were involved.There were 3410 genes involved in liver cirrhosis,and 103 overlapping genes between the two genes.It may mediate positive regulation of tumor necrosis factor related apoptosis inducing ligand activated apoptotic signaling pathways,positive regulation of iron on translation initiation,and inactivation of mitogen activated protein kinase K activity targets in cirrhosis,and 25 pathways involved in hypoxia inducible factor 1 signaling pathway,Prolactin signaling pathway and forkhead box O signaling pathway in liver cirrhosis(P≤0.01).Conclusion The network pharmacology can be used to preliminarily predict the potential targets of the treatment of cirrhosis by Rhizoma Anemarrhenae of Miao medicine,which provides scientific basis for the further study of the pharmacological mechanism.

关 键 词：肝硬化 苗药土知母 分子机制 网络药理学 

分 类 号：R932[医药卫生—生药学]