不同釉质黏结剂对牙龈卟啉单胞菌和变形链球菌的影响  

Effects of enamel adhesives with different components on Porphyromonas gingivalis and Streptococcus mutans

作　　者：李志耀 胡铮[2] 李晅 陆珮珺[2] Li Zhiyao;Hu Zheng;Li Xuan;Lu Peijun(Department of Oral Surgery,Shanghai Xuhui District Dental Center,Shanghai 200032,China;Department of Orthodontics,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,College of Stomatology,Shanghai Jiao Tong University,National Center for Stomatology,National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology,Shanghai Research Institute of Stomatology,Shanghai 200011,China;Department of Orthodontics,Shanghai Stomatological Hospital,Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases,Shanghai 200001,China;Department of Stomatology,Children’s Hospital of Fudan University,Shanghai 201103,China)

机构地区：[1]上海市徐汇区牙病防治所口腔外科,上海市200032 [2]上海交通大学医学院附属第九人民医院口腔正畸科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海市200011 [3]上海市口腔医院口腔正畸科,上海市颅颌面发育与疾病重点实验室,上海市200001 [4]复旦大学附属儿科医院口腔科,上海市201103

出　　处：《中国组织工程研究》2024年第3期329-335,共7页Chinese Journal of Tissue Engineering Research

基　　金：上海市卫生局青年基金项目(20144Y0219),项目负责人:李晅。

摘　　要：背景:光固化型和含氟光固化型釉质黏结剂对酸蚀后的牙釉质表面有一定封闭作用,含氟光固化型釉质黏结剂还能通过释放氟离子实现防龋功能,但缺乏对氟化物较长时间释放的研究,尤其缺乏经历1-3个月氟化物释放后局部致病菌量的变化信息。目的:分析青少年正畸治疗患者上前牙使用不同组分釉质黏结剂黏结固定矫治器引发的龈下菌斑中牙龈卟啉单胞菌和变形链球菌表达变化。方法:选择2016年1-12月就诊于上海市口腔医院接受正畸治疗的青少年患者90例,其中男43例,女47例,平均年龄(13.27±1.12)岁。采用随机数字表法将90例患者分为3组:化学固化组应用Unite™黏结树脂进行固定矫治器的黏结,光固化组应用Transbond XT光固化树脂进行固定矫治器的黏结,含氟光固化组应用GC光固化正畸黏结用水门汀进行固定矫治器的黏结,每组30例。在黏结托槽当天(初始黏结)及第1,2,3个月复诊时收集患者上前牙龈下菌斑标本,应用PCR技术检测牙龈卟啉单胞菌和变形链球菌的增殖表达量。结果与结论:①组内比较:随着黏结时间的增加,化学固化组3个月复诊时的牙龈卟啉单胞菌增殖表达显著高于初始黏结时(P<0.05);光固化组1个月复诊时的牙龈卟啉单胞菌增殖表达低于初始黏结时(P<0.05);含氟光固化组1,2个月复诊时的牙龈卟啉单胞菌增殖表达均低于初始黏结时(P<0.05)。化学固化组复诊1,2,3个月时的变形链球菌表达高于初始黏结时(P<0.05);含氟光固化组复诊1,2,3个月时的变形链球菌增殖表达均低于初始黏结时(P<0.05);光固化组复诊时的变形链球菌增殖表达与初始黏结时比较差异无显著性意义(P>0.05);②组间比较:1个月复诊时,光固化组、含氟光固化组牙龈卟啉单胞菌增殖表达低于化学固化组(P<0.05);2,3个月复诊时,含氟光固化组牙龈卟啉单胞菌增殖表达低于光固化组、化学固化组(P<0.05)。1,2,3�BACKGROUND:The light curing and fluoride light curing enamel adhesives have a certain sealing effect on the etched enamel surface.The fluoride light curing enamel adhesives can also achieve the anti-caries function by releasing fluoride ions.However,the existing researches lack the long-term tracing of fluoride release effect,especially the amount of local pathogenic bacteria after 1-3 months of local fluoride application.OBJECTIVE:To analyze the changes in the expression of Porphyromonas gingivalis and Streptococcus mutans in subgingival plaque of the upper anterior teeth adhered by different components of enamel adhesives in adolescent patients with fixed appliance.METHODS:Ninety adolescent patients who received orthodontic treatment in Shanghai Stomatological Hospital from January to December 2016 were enrolled,including 43 males and 47 females,with a mean age of(13.27±1.12)years.These patients were randomly divided into three groups(n=30 per group).In the chemical curing group,Unite™bonding resin was used to bond fixed appliances.In the light curing group,Transbond XT light curing resin was used to bond the fixed appliance.In the fluoride light curing group,GC light curing orthodontic adhesive was used to bond the fixed appliance with cement.The subgingival plaque was collected on the day of bonding,1st,2nd,and 3rd month follow-up reviews.The expressions of Porphyromonas gingivalis and Streptococcus mutans in subgingival plaque were detected by PCR.RESULTS AND CONCLUSION:(1)Intragroup comparison:With the increase of bonding time,Porphyromonas gingivalis expression increased significantly in the 3rd month in the chemical curing group(P<0.05).In the light curing group,Porphyromonas gingivalis showed a significant decrease in the 1st month(P<0.05).Porphyromonas gingivalis expression decreased significantly in the 1st and 2nd months compared with initial data in the fluoride light curing group(P<0.05).The expression of Streptococcus mutans was higher in the chemical curing group in the 1st,2nd,and 3rd months c

关 键 词：口腔正畸 固定矫治器 釉质黏结剂 牙龈卟啉单胞菌 变形链球菌 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学] R781.4