明胶/氧化纳米纤维素高弹性模量高孔隙皮肤支架的3D打印工艺  

3D printing process of gelatin/oxidized nanocellulose skin scaffold with high elastic modulus and high porosity

作　　者：许晓东[1,2] 周骥平 张琦[3] 冯辰[1,2] 朱勉顺 史宏灿 Xu Xiaodong;Zhou Jiping;Zhang Qi;Feng Chen;Zhu Mianshun;Shi Hongcan(Yangzhou Polytechnic Institute,Yangzhou 225127,Jiangsu Province,China;Jiangsu Province Engineering Research Center of Intelligent Application for Advanced Plastic Forming,Yangzhou 225127,Jiangsu Province,China;College of Mechanical Engineering,Yangzhou University,Yangzhou 225127,Jiangsu Province,China;Medical College,Yangzhou University,Yangzhou 225009,Jiangsu Province,China)

机构地区：[1]扬州工业职业技术学院,江苏省扬州市225127 [2]江苏省新材料塑性成形智能化应用工程研究中心,江苏省扬州市225127 [3]扬州大学机械工程学院,江苏省扬州市225127 [4]扬州大学医学院,江苏省扬州市225009

出　　处：《中国组织工程研究》2024年第3期398-403,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81770018),项目负责人:史宏灿;扬州市-扬州大学科技合作项目(SCX2017020015),项目负责人:周骥平;江苏省高等学校基础科学(自然科学)研究面上项目(21KJD460010),项目负责人:许晓东;2021年度校级科研课题(自然科学)项目(2021xjzk003),项目负责人:许晓东。

摘　　要：背景:在采用主动修复治疗手段应对皮肤创伤时,需要使用组织工程技术生成新的组织来代替坏死组织,皮肤支架在创伤修复领域具有良好的应用前景。皮肤支架需要呈现具有一定力学强度的三维多孔结构,以满足细胞增殖分裂的需求,而目前使用的明胶基生物材料力学强度弱,无法达到皮肤支架的使用要求。目的:针对明胶/氧化纳米纤维素复合材料制备组织工程皮肤支架时使用的3D打印工艺进行研究,重点研究不同工艺参数下制备皮肤支架的孔隙率与其力学强度之间的关系。方法:从葎草中提取10%浓度的氧化纳米纤维素晶须,再与5%的明胶复合得到明胶/氧化纳米纤维素复合材料,检测明胶与明胶/氧化纳米纤维素复合材料的弹性模量。以明胶/氧化纳米纤维素复合材料为基材,采用3D打印挤压成型方法制备皮肤支架,通过对材料进行力学性能测试和流变特性测试确定挤压成型工艺参数(填充间隙1.5-2.5 mm,0.1 mm均布;气压160-200 kPa),并以此制备具有三维多孔结构的皮肤支架。对皮肤支架进行了抗压性能的测试并与有限元分析结果相对比,论证了支架打印时的填充间隙与支架孔隙率及力学强度之间的关系。结果与结论:①通过实验得出,加入10%浓度的氧化纳米纤维素晶须使5%明胶的弹性模量度提升了8.84倍;在气压160 kPa下挤出成型可以得到1 mm直径的丝状凝胶,此时填充间隙从1.5 mm增大到2.5 mm会使支架的理论孔隙率从33%上升到60%,但抗压强度从230000 Pa降低到95000 Pa;②结果显示,使用2 mm填充间隙制备得到了理论孔隙率为50%、弹性模量160000 Pa的皮肤支架,该支架具有清晰的三维多孔结构。BACKGROUND:In the treatment of skin trauma with active repair,tissue engineering techniques are needed to generate new tissue to replace necrotic tissue.Skin scaffolds have a good application prospect in the field of wound repair.Skin scaffolds need to present three-dimensional porous structures with certain mechanical strength to meet the needs of cell proliferation and division.However,the mechanical strength of the currently used gelatin-based biomaterials is weak and cannot meet the requirements of the use of skin scaffolds.OBJECTIVE:To study the 3D printing process used in the preparation of tissue engineering skin scaffolds by gelatin/oxidized nanocellulose composites,and focus on the relationship between the porosity and mechanical strength of the scaffolds prepared under different process parameters.METHODS:Oxidized nanocellulose whiskers at 10%concentration were extracted from Humulus scandens and then compounded with 5%gelatin to obtain gelatin/oxidized nanocellulose composites.The elastic modulus of gelatin and gelatin/oxidized nanocellulose composite was determined.Skin scaffolds were prepared by 3D printing extrusion molding using gelatin/oxidized nanocellulose composite as the base material.Mechanical and rheological properties of the composite were tested to determine extrusion molding parameters(filling gap 1.5-2.5 mm,uniform distribution of 0.1 mm;air pressure of 160-200 kPa),and the skin scaffold with a three-dimensional porous structure was prepared.The compressive performance of the skin scaffold was tested and compared with the finite element analysis results.The relationship between the filling gap and the porosity and mechanical strength of the scaffold was demonstrated.RESULTS AND CONCLUSION:(1)The elastic modulus of 5%gelatin was increased by 8.84 times by adding 10%oxidized nanocellulose whisker.A gel filament with a diameter of 1 mm was obtained by extrusion at the air pressure of 160 kPa.When the filling gap increased from 1.5 mm to 2.5 mm,the theoretical porosity of the scaffold incre

关 键 词：皮肤支架 弹性模量 孔隙率 3D打印 填充间隙 氧化纳米纤维素 复合凝胶 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R622.1