骨组织工程中研究水凝胶微球的特征  

Characteristics of hydrogel microspheres in bone tissue engineering

作　　者：龙智睿 黄雷[1] 肖放 王琳[1,2] 王晓蓓 Long Zhirui;Huang Lei;Xiao Fang;Wang Lin;Wang Xiaobei(Tissue Engineering and Regenerative Medicine Research Center,Union Hospital Affiliated to Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,Hubei Province,China;Department of Clinical Laboratory,Union Hospital Affiliated to Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,Hubei Province,China)

机构地区：[1]华中科技大学同济医学院附属协和医院组织工程与再生医学研究中心,湖北省武汉市430022 [2]华中科技大学同济医学院附属协和医院检验科,湖北省武汉市430022

出　　处：《中国组织工程研究》2024年第3期472-478,共7页Chinese Journal of Tissue Engineering Research

摘　　要：背景:水凝胶微球由于其多孔性和可注射性等在递送细胞和生物活性因子/药物、构建组织修复支架等生物医学领域展现独特优势,具有广阔的应用前景。目的:综述基于水凝胶微球的骨组织工程最新研究进展,讨论基于水凝胶微球的骨组织工程研究面临的关键问题和挑战。方法:应用计算机检索PubMed和中国知网数据库收录的相关文献。英文检索词为“hydrogels,microparticles,microspheres,microcarriers,bone,bone defect,bone repair,bone healing,bone tissue engineering”,中文检索词为“水凝胶,微球,微粒,骨组织工程,骨缺损,骨修复,骨再生”。检索文献时限为2002-2022年,最终纳入127篇文献进行综述。结果与结论:①目前,不同的水凝胶微球材料已被开发用于骨组织工程策略并取得了较好的效果,如搭载细胞或生物活性因子/药物的水凝胶微球、作为生物支架的水凝胶微球、刺激响应性水凝胶微球、生物矿化水凝胶微球、与其他生物材料结合的水凝胶微球等。②基于水凝胶微球的骨组织工程修复策略主要通过促进干细胞的招募与成骨分化、调节损伤局部炎症微环境以及促进损伤部位血管生成等机制来调控骨修复。但目前的研究没有深入探索基于水凝胶微球的骨组织工程诱导内源性干细胞招募与分化,以及水凝胶微球的理化性质对炎症微环境的调控,且水凝胶微球的体内长期不良反应尚未探明,批量生产存在困难,因此,未来的研究需要在机制探索和技术路线上加强深入,从而为开发能够用于临床转化的水凝胶微球材料提供合理参考。BACKGROUND:Hydrogel microparticles,due to their porous and injectable properties,have demonstrated unique advantages in biomedical fields,such as the delivery of cells and bioactive factors/drugs,the construction of tissue repair scaffolds.They have broad application prospects.OBJECTIVE:To review the latest research progress and discuss the key problems and challenges in the research of bone tissue engineering based on hydrogel microparticles.METHODS:The relevant articles in PubMed and CNKI were searched by computer.The English key words were“hydrogels,microparticles,microspheres,microcarriers,bone,bone defect,bone repair,bone healing,bone tissue engineering”while the Chinese key words were“hydrogels,microparticles,microspheres,bone tissue engineering,bone defect,bone repair,bone regeneration”.The retrieval period was from 2002 to 2022,and 127 articles were finally included for review.RESULTS AND CONCLUSION:(1)At present,various hydrogel microparticles have been developed for use in bone tissue engineering strategies,for example,hydrogel microparticles carrying cells or bioactive factors/drugs,hydrogel microparticles as biological scaffolds,stimulus-responsive hydrogel microparticles,biomineralized hydrogel microparticles,hydrogel microparticles combined with other biological materials.(2)Bone tissue engineering repair strategies based on hydrogel microparticles mainly regulate bone repair by promoting stem cell recruitment and osteogenic differentiation,regulating the local inflammatory microenvironment and promoting angiogenesis at the site of injury.However,the present studies did not deeply explore the effect of bone tissue engineering based on hydrogel microparticles on the recruitment and differentiation of endogenous stem cells and the regulation of the inflammatory microenvironment by the physical and chemical properties of hydrogel microparticles.The long-term in vivo adverse reactions of hydrogel microparticles have not been explored yet,and it is difficult to mass-produce them,thus future resea

关 键 词：水凝胶微球 球形水凝胶 骨缺损 骨修复 骨再生 骨组织工程 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学] R-1