人参多糖干预创伤性骨关节炎模型大鼠前列腺素E_(2)/6-酮-前列腺素F_(1α)的表达  

Effect of ginseng polysaccharide on the expression of prostaglandin E_(2)/6-keto-prostaglandin 1alpha in traumatic osteoarthritis model rats

作　　者：李蕊 张桂红 王涛[2] 樊萍[3] Li Rui;Zhang Guihong;Wang Tao;Fan Ping(Shaanxi Vocational and Technical College of Energy,Xianyang 712000,Shaanxi Province,China;Shaanxi Provincial People’s Hospital,Xi’an 710007,Shaanxi Province,China;The First Affiliated Hospital of Xi’an Jiaotong University School of Medicine,Xi’an 710007,Shaanxi Province,China)

机构地区：[1]陕西能源职业技术学院,陕西省咸阳市712000 [2]陕西省人民医院,陕西省西安市710007 [3]西安交通大学医学院第一附属医院,陕西省西安市710007

出　　处：《中国组织工程研究》2024年第8期1235-1240,共6页Chinese Journal of Tissue Engineering Research

基　　金：陕西省国际科技合作与交流计划项目(2016KW-023),项目负责人:樊萍。

摘　　要：背景:目前已有研究发现植物人参提取物对骨关节炎有明显的改善作用,但是关于人参多糖提对骨关节炎的治疗作用尚未见报道。目的:探讨人参多糖干预创伤性骨关节炎模型大鼠前列腺素E_(2)/6-酮-前列腺素F_(1α)的表达变化。方法:选取60只雄性SD大鼠,随机分为健康组、模型组、人参多糖低、中、高剂量组、地塞米松组,除健康组外,其余大鼠均建立创伤性骨关节炎模型。造模成功后,健康组与模型组采用生理盐水0.2 mL腹腔注射,人参多糖低、中、高剂量组分别采用0.1,0.25,0.5μg/mL人参多糖0.2 mL腹腔注射,地塞米松组采用0.2 mg/kg地塞米松腹腔注射,均每3 d注射一次,连续干预4周。给药结束后采用ELISA法检测大鼠血清中前列腺素E_(2)、6-酮-前列腺素F_(1α)水平,Mankin’s评分法检测大鼠膝关节软骨功能,苏木精-伊红染色观察大鼠膝关节病理形态,免疫印迹与PCR分别检测关节软骨组织中肿瘤坏死因子α、白细胞介素1β、白细胞介素10的表达。结果与结论:①与模型组比较,人参多糖中剂量组、地塞米松组大鼠血清前列腺素E_(2)降低,6-酮-前列腺素F_(1α)升高(P<0.05);与人参多糖中剂量组、地塞米松组比较,人参多糖高剂量组大鼠上述指标显著改善(P<0.05);人参多糖中剂量组及地塞米松组无差异(P>0.05);②与模型组比较,人参多糖中剂量组、地塞米松组大鼠Mankin’s评分降低(P<0.05);与人参多糖中剂量组、地塞米松组比较,人参多糖高剂量组Mankin’s评分显著降低(P<0.05);人参多糖中剂量组及地塞米松组无差异(P>0.05);③模型组与人参多糖低剂量组大鼠软骨组织层明显变薄,深达骨质层的裂隙及软骨细胞大量丢失,潮线严重断裂、模糊,滑膜层胶原纤维增多、增粗,可见大量软骨细胞被破坏,排列不规则;人参多糖中剂量组、地塞米松组较模型组改善;人参多糖高剂量组较人参多糖中剂量组改善BACKGROUND:Ginseng extracts have been found to significantly improve osteoarthritis,but the therapeutic effects of ginseng polysaccharide extracts on osteoarthritis have not been reported.OBJECTIVE:To investigate the effect of ginseng polysaccharide on the expression of prostaglandin E_(2)/6-keto-prostaglandin F_(1α)in traumatic osteoarthritis model rats.METHODS:Sixty male Sprague-Dawley rats were selected and randomly divided into healthy group,model group,ginseng polysaccharide low-dose group,ginseng polysaccharide medium-dose group,ginseng polysaccharide high-dose group and dexamethasone group.Except for 10 rats in the healthy group,the other rats were taken to establish traumatic osteoarthritis models.The healthy group and model group were given 0.2 mL of normal saline intraperitoneally.The low-,medium-,and high-dose groups were intraperitoneally injected with 0.1,0.25,0.5μg/mL ginseng polysaccharide,respectively.In the dexamethasone group,0.2mg/kg dexamethasone(0.2 mL)was injected intraperitoneally.Injections were given once every 3 days,for 4 consecutive weeks.Serum prostaglandin E_(2) and 6-keto-prostaglandin F_(1α)levels were detected by ELISA.The bone and joint function of rats were assessed by the Mankin’s score.Hematoxylin-eosin staining was used to observe the pathologic morphology of the knee joints of rats.Western blot and PCR were used to detect the protein and mRNA expression of tumor necrosis factorαand interleukin-1β,interleukin-10 in articular cartilage tissue,respectively.RESULTS AND CONCLUSION:Compared with the model group,serum prostaglandin E_(2) levels were decreased in the medium-dose group and dexamethasone group,while serum 6-keto-prostaglandin F_(1α)levels were increased(P<0.05).Compared with the medium-dose group and dexamethasone group,the abovementioned indicators were significantly improved in the high-dose group,and there was no significant difference between the medium-dose group and dexamethasone group(P>0.05).Compared with the model group,the Mankin’s score was reduce

关 键 词：人参多糖 创伤性骨关节炎 前列腺素E_2 6-酮-前列腺素F_(1α) 动物模型 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R365