黑龙江省哈尔滨市苜蓿镰孢根腐病病原菌分离鉴定  

Isolation and identification of pathogens of Fusarium root rot on alfalfa in Harbin City,Heilongjiang Province

作　　者：朱月 任乃芃 唐佳琳 刘香萍[1] ZHU Yue;REN Nai-peng;TANG Jia-lin;LIU Xiang-ping(College of Animal Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《草原与草坪》2023年第2期99-106,共8页Grassland and Turf

基　　金：黑龙江省科技计划省院科技合作项目(YS20B10);黑龙江八一农垦大学研究生创新科研项目(YJSCX2021-Y01);黑龙江八一农垦大学学成、引进人才科研启动计划(XDB2014-05)、大庆市新能源领域“揭榜挂帅”科技攻关项目(2021BD05)。

摘　　要：【目的】明确黑龙江省哈尔滨市苜蓿根腐病病原菌种类。【方法】采集38株黑龙江省哈尔滨市农业科学院示范区的苜蓿根腐病病株,经组织分离法分离致病菌,通过科赫氏法则验证其致病性并结合形态学和rDNA-ITS序列分析鉴定病原菌。【结果】从患有苜蓿根腐病的病根分离并选取具有代表性的5株真菌KY1、J1、S1、SA1、C1,分别被鉴定为腐皮镰孢(Fusarium solani)、尖镰孢(F.oxyspo⁃rum)、水稻恶苗病菌(F.fujikuroi)、三线镰孢(F.tricinctum)和层出镰孢(Fusariumproliferatum)。致病性测定表明5株真菌均为苜蓿根腐病致病菌。【结论】腐皮镰孢不仅分离率最高,为57.45%,且致病性也较强,病情指数为61.00,是优势病原菌。【Objective】To clarify the pathogenic species of Fusarium root rot of alfalfa in Harbin City,Heilongji⁃ang Province.【Method】Thirty⁃eight alfalfa root rot disease strains were collected from the demonstration area of Harbin Academy of Agricultural Sciences in Heilongjiang Province,and the pathogenic fungi were isolated by tissue separation method,verified by Koch's rule and identified by morphology and rDNA⁃ITS sequence analysis.【Result】The five representative fungal strains,KY1,J1,S1,SA1 and C1,were isolated and selected from alfalfa roots and identified as F.solani,F.oxysporum,F.fujikuroi,F.tricinctum and F.proliferatum,respectively.The pathogenicity test showed that all the five fungi were pathogenicfor alfalfa root rot.【Conclusion】F.solani not only had the highest isolation rate(57.45%),but also had strong pathogenicity,with a disease index of 61.00,which was the dominant pathogen.The alfalfa root rot caused by F.fujikuroi was reported for the first time in China.

关 键 词：苜蓿 根腐病 腐皮镰孢 鉴定 致病性 

分 类 号：S541.1[农业科学—作物学]