香棕根际土一株拮抗病原真菌细菌的分离鉴定及生物学性质研究  

Isolation and Identification of an Antagonistic Fungal Bacterium from the Rhizosphere Soil of Arenga Engleri and Research of their Biological Characteristics

作　　者：吕祎凡 周瑞鹏 陈星烨 赖泽成 张春源 邵登科 刘梓烨 陈诗瑜 李望豪 叶文雨[1,2] LV Yi-fan;ZHOU Rui-peng;CHEN Xing-ye;LAI Ze-cheng;ZHANG Chun-yuan;SHAO Deng-ke;LIU Zi-ye;CHEN Shi-yu;LI Wang-hao;YE Wen-yu(China National Engineering Research Center of Juncao Technology,Fuzhou Fujian 350002,China;Fujian University Key Laboratory for Plant-Microbe Interaction,College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou Fujian 350002,China;College of Future Technology(Haixia Institute of Science and Technology),Fujian Agriculture and Forestry University,Fuzhou Fujian 350002,China)

机构地区：[1]福建农林大学国家菌草工程技术研究中心,福建福州350002 [2]植物与微生物相互作用福建省高校重点实验室,福建农林大学生命科学学院,福建福州350002 [3]福建农林大学未来技术学院(海峡联合研究院),福建福州350002

出　　处：《青海农林科技》2023年第2期1-6,共6页Science and Technology of Qinghai Agriculture and Forestry

基　　金：福建农林大学科技创新专项基金项目(No.KFA19137A);福建农林大学科技创新专项基金项目(No.KFA19136A);福建省自然资源科技创新项目资金资助(Y-090000-04-2022-016);福建省科技重大专项(2021NZ029009);福建省引导性科技计划项目(2022N0010);大学生创新训练项目(S202210389072)。

摘　　要：本研究以海南文昌香棕根际土作为材料,分离得到一种土壤细菌,编号为HNXZB1。菌株HNXZB1的形态学、16S rDNA分子鉴定结果均为炭疽杆菌,革兰氏染色结果为阳性,且具有降解淀粉、分解无机磷、分解糖产生丙酮酸、产生过氧化氢酶的能力。拮抗能力试验结果表明,该细菌对于稻瘟病菌和禾谷镰刀菌均有拮抗作用,抑制率分别达到53.53%,49.38%。本研究将为植物根际细菌功能研究提供参考,并为开发抗菌剂或生物有机肥提供理论指导。This study isolated a soil bacterium named HNXZB1 from the rhizosphere soil of Hainan Wenchang Arenga engleri.The morphological and 16S rDNA molecular identification results of strain HNXZB1 were all Bacillus anthracis,with a positive Gram staining result.It has the ability to degrade starch,decompose inorganic phosphorus,decompose sugar to produce pyruvate,and produce catalase.The results of antagonistic ability test showed that the bacteria had antagonism against Magnaporthe oryzae and Fusarium graminearum,and the inhibition rates were 53.53%and 49.38%,respectively.This study will provide reference for the functional research of plant rhizosphere bacteria and theoretical guidance for the development of antibacterial agents or bio-organic fertilizers.

关 键 词：香棕 根际土细菌 理化性质 拮抗病原真菌 

分 类 号：S182[农业科学—农业基础科学]