Real-Time and Spatial Electroanalysis of Biomolecules in One Living Cell Using Liquid-Phase Modified Nanopipette  

作　　者：Kang Liu Rujia Liu Dengchao Wang Rongrong Pan Hong-Yuan Chen Dechen Jiang 

机构地区：[1]The State Key Laboratory of Analytical Chemistry for Life Sciences,School of Chemistry and Chemical Engineering,Chemistry and Biomedicine Innovation Center(ChemBIC),Nanjing University,Nanjing,Jiangsu 210093 [2]School of Chemical Sciences,University of Chinese Academy of Science,Beijing 100190

出　　处：《CCS Chemistry》2023年第6期1285-1292,共8页中国化学会会刊（英文）

基　　金：This work was supported by the Ministry of Science and Technology of China(grant no.2017YFA0700500);the National Natural Science Foundation of China(grant nos.22025403,22104051,and 21974060);the Jiangsu Social Development Project(grant no.BE2022794);the China Postdoctoral Science Foundation(grant no.2021M691507);Yuxiu Young Scholars Program(2020);the Scientific Research Support Program for Postdoctoral Fellows of Jiangsu Province(grant no.2021K098A).

摘　　要：The well-developed solid-phase modified strategy at the electrode has enabled the preparation of biosensors for the detection of multiple analytes,even in single living cells.However,limited assay elements can be modified at the solid surface,restricting the types of molecules that can be analyzed and the sensitivity of detection.Here,a novel liquid-phase modified strategy at the tip of a nanopipette is designed to realize real-time and local analysis of biomolecules inside the cell that are barely detectable using solid-phase modified nanoelectrodes.This design utilizes the nanotip structure at a platinized carbon open nanopipette to stably retain a nanodroplet that contains the required reagents with high reactivity for the assay of the target analyte.The generated hydrogen peroxide is electrochemically quantified at the Pt layer to carry out the real-time measurement in a living cell with a spatial resolution of 70 nm.Taking advantage of highly spatial and real-time detection,uneven distribution of sphingomyelinase(SMase)in the living CT26 cell is unprecedentedly shown to exhibit the significance in the establishment of liquid-phase modified nanopipette.This new modification strategy opens up a new direction for sensor design and consequently advances the development of biosensors in the chemical and biological research.

关 键 词：liquid-phase modified nanoelectrode local analysis of biomolecules NANODROPLET spatial information of SMase 

分 类 号：O62[理学—有机化学]