通过网络药理学探讨山豆根干预肺癌的作用机制及物质基础  

Action mechanism and material basis of Sophora tonkinensis Gagnep in the treatment of lung cancer by network pharmacology

作　　者：何雅风 黄华进 李红美 张帅男 李煦照 HE Yafeng;HUANG Huajin;LI Hongmei;ZHANG Shuainan;LI Xuzhao(Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China)

机构地区：[1]贵州中医药大学,贵州贵阳550025

出　　处：《贵州科学》2023年第3期6-11,共6页Guizhou Science

基　　金：国家自然科学基金项目(8196140426)。

摘　　要：目的:研究山豆根(Sophora tonkinensis Gagnep)治疗肺癌的物质基础和作用机制。方法:首先对山豆根进行液相色谱-质谱联用分析,获得有效成分,随后通过数据库Pubchem,获得山豆根成分的2D结构,然后将这些2D结构导入到Swiss Target Prediction数据库,预测其作用靶点。在CTD数据库中,查找出与肺癌相关的靶点,采用韦恩图对山豆根化学成分的靶点与其肺癌相关靶点进行结合,得出二者相互交叉的部分,即为交集靶点。采用Cytoscape v3.8.0软件构建“成分-靶点-疾病”的网络关系,采用DAVID数据库对交集靶点进行KEGG通路分析。最后对交集靶点进一步分析,获得山豆根治疗肺癌的有效成分。结果:获得44个交集靶点,进一步挑选出核心靶点5个,筛选出8条KEGG信号通路和8个山豆根治疗肺癌的活性成分。结论:本文对山豆根具有多成分、多靶点、多通路等特点的肺癌治疗方法进行了研究和分析,为今后的肺癌治疗提供了理论依据。In this study we analyzed the material basis and action mechanism of Sophora tonkinensis Gagnep in the treatment of lung cancer.Firstly,liquid chromatography-mass spectrometry was used to analyze the active components of Sophora tonkinensis.Then the 2D structures of the active components were attained by using the PubChem database,and these 2D structures were imported into the Swiss Target Prediction database to predict their action targets.In the CTD database,the targets related to lung cancer were found.The Venn diagram was used to combine the active components of Sophora tonkinensis with the lung cancer related targets,and the intersection targets were obtained.Cytoscape v3.8.0 software was used to construct the component-target-disease network,and the DAVID database was used to analyze the KEGG pathways of the intersection targets.Finally,the intersection targets were further analyzed to attain the effective components of Sophora tonkinensis in the treatment of lung cancer.A total of forty-four intersection targets were attained,five core targets were further selected,eight KEGG signal pathways were screened out,and eight active components of Sophora tonkinensis in the treatment of lung cancer were obtained.This study has provided a theoretical basis for the treatment of lung cancer in the future.

关 键 词：网络药理学 山豆根 肺癌 物质基础 作用机制 靶点 

分 类 号：R284.1[医药卫生—中药学]