单间作小麦响应白粉病菌侵染的差异代谢物及代谢通路  

Analysis of differential metabolites and metabolic pathways of mono-and intercropped wheat in response to Blumeria graminis f.sp.tritici infection

作　　者：陈升 吴鑫雨 何建杨 周懂 刘振洋 汤利[1] 郑毅[1,2] 肖靖秀 CHEN Sheng;WU Xinyu;HE Jianyang;ZHOU Dong;LIU Zhenyang;TANG Li;ZHENG Yi;XIAO Jingxiu(College of Resources and Environment,Yunnan Agricultural University,Kunming 650201,China;Yunnan Open University,Kunming 650233,China)

机构地区：[1]云南农业大学资源与环境学院,昆明650201 [2]云南开放大学,昆明650233

出　　处：《中国生态农业学报（中英文）》2023年第6期904-916,共13页Chinese Journal of Eco-Agriculture

基　　金：国家自然科学基金项目(32060718,31760611);云南省高层次人才培养支持计划“青年拔尖人才”项目(YNWR-QNBJ-2019-130)资助。

摘　　要：为了解单作、间作小麦响应白粉病侵染的代谢差异,揭示间作提高小麦抗白粉病的生理机制,本文通过盆栽试验设置75 mg·kg^(-1)(N1)、150 mg·kg^(-1)(N2)、225 mg·kg^(-1)(N3)3个施氮水平,研究接种白粉病病原菌后,小麦单一种植和小麦蚕豆间作下白粉病的发病情况,并通过广泛靶向代谢组学分析单作、间作小麦响应白粉病菌侵染的差异。结果表明:氮水平和氮水平×种植模式显著影响小麦白粉病发病率和病情指数;在3个氮水平下,间作降低白粉病发病率25.54%~38.81%、降低病情指数20.11%~21.97%,其中低氮水平控制效果较好。白粉病菌侵染后,单作、间作小麦叶片中共检测到822种代谢产物,N1、N2和N3水平下分别发现差异代谢物69种、52种和88种。KEGG代谢通路分析发现单间作小麦差异代谢物主要富集在氨基酸的生物合成、代谢途径和次生代谢物的生物合成。其中N1和N2水平下,差异代谢物富集在代谢途径,N1和N3水平下差异代谢物富集于氨基酸的生物合成。进一步对上调、下调差异倍数前10的代谢物分析发现,与单作相比,N1水平间作上调了谷胱甘肽还原型、L-色氨酸、L-天冬酰胺和L-谷氨酰胺,N3水平间作上调了L-天冬酰胺、L-高甲硫氨酸和L-色氨酸。除此之外,少数生物碱类、酚酸类和有机酸类等代谢物质在氮胁迫下也呈现不同程度的变化。总之,单作和间作小麦响应白粉病病菌侵染的应答过程受氮水平调控。在白粉病病菌侵染中,间作调控差异代谢物如氨基酸及其衍生物类、生物碱类、酚酸类和有机酸类等在植物体内的变化,可能是间作提高小麦白粉病抗性的机制之一。其中,氮胁迫条件下间作调控氨基酸及其衍生物与小麦白粉病抗性提高密切相关。Wheat and faba bean intercropping can alleviate the occurrence and severity of wheat powdery mildew.However,the physiological mechanism of intercropping improving wheat disease resistance remains unclear.This study aimed to understand the metabolic differences between mono-and inter-cropped wheat in response to Blumeria graminis f.sp.tritici infection and reveal the physiological mechanism of intercropping for improving wheat resistance to powdery mildew.In this study,the following three nitrogen(N)application levels were established:75 mg·kg^(-1)(N1),150 mg·kg^(-1)(N2),and 225 mg·kg^(-1)(N3).Following inoculation with B.graminis f.sp.tritici,the occurrence of powdery mildew in mono-and inter-cropped wheat was investigated,and the metabolomics of mono-and inter-cropped wheat in response to infection with B.graminis f.sp.tritici were analyzed by UPLC-MS/MS,using a widely targeted metabolomic method.The results showed that N levels and N levels×planting patterns significantly affected the incidence and disease indices of powdery mildew in wheat.Under all three N levels,wheat intercropping with faba bean reduced the incidence of wheat powdery mildew by 25.54%–38.81%and decreased the disease index by 20.11%–21.97%relative to mono-cropped wheat(MW),and the intercropping control effect under the N1 level was better than that under N2 and N3 conditions.A total of 822 differential metabolites were detected in the mono-and inter-cropped wheat leaves,of which 69,52,and 88 were detected at the N1,N2,and N3 levels,respectively.Intercropping of wheat and faba bean regulated flavonoids,alkaloids,amino acids and derivatives,and phenolic acids in wheat leaves compared to MW.Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of differential metabolites showed that they were mainly enriched in the biosynthesis of amino acids,metabolic pathways,and secondary metabolites.Among them,metabolites with significant differences were enriched in metabolic pathways at the N1 and N2 levels,and metabolites with signif

关 键 词：小麦蚕豆间作 小麦白粉病 氮水平 差异代谢物 

分 类 号：S512.1[农业科学—作物学]