杨树MYC基因家族成员表达模式研究  

Analysis of Expression Patterns of MYC Family Members in Populus

作　　者：胡梦璇 宋学勤[1] 刘颖丽[1] 赵树堂[1] HU Meng-xuan;SONG Xue-qin;LIU Ying-li;ZHAO Shu-tang(State Key Laboratory of Tree Genetics and Breeding,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China)

机构地区：[1]林木遗传育种全国重点实验室,中国林业科学研究院林业研究所,北京100091

出　　处：《林业科学研究》2023年第3期32-40,共9页Forest Research

基　　金：国家自然科学基金面上项目(32271902)。

摘　　要：[目的]探究bHLH亚家族成员MYC基因在杨树生长发育及对环境响应中的表达模式,为解析茉莉酸信号通路关键基因MYC调控杨树生长发育及抗逆响应提供参考。[方法]利用生物信息学方法鉴定杨树基因组中的MYC基因家族成员并对各成员的基因结构、保守基序进行了系统分析;在此基础上,采用实时荧光定量PCR技术检测了MYC成员在不同组织、不同植物激素响应及逆境胁迫下的表达模式。[结果]毛果杨基因组中含有10个MYC成员,这些成员在进化上相对保守,均具有典型的bHLH结构域,根据进化关系将其分为3个分支。组织表达分析发现,大部分MYC家族成员在根中均具有较高表达量,分枝Ⅱ两对基因在茎中呈相反表达模式。不同激素处理及非生物胁迫条件下MYC基因存在显著表达差异,但基因对之间多存在类似的表达模式。[结论]杨树MYC基因家族成员可能参与不同的生物学过程,研究结果为深入解析杨树MYC基因的功能奠定了基础。[Objective]To explore the expression patterns of MYC genes,a subfamily of bHLH gene family,in the growth and development of poplar,and the response to different hormones and environmental factors,provides references for revealing the roles of MYCs in regulation of plant growth and environmental adaptability.[Method]Bioinformatic analysis was used to determine the family members of MYC and their gene structure and conserved motifs.Quantitative real-time PCR was carried out to analyze the expression patterns of MYC family members in different tissues and under different kinds of phytohormones and stress treatments.[Result]Ten MYC members was found in the genome of Populus and the PtrMYC genes were conserved during evolution,and all of them contained bHLH domains,which were divided into three classes in phylogenetic tree.Analysis of the expression patterns showed that most members were mainly expressed in the roots,and two gene pairs in cladeⅡhad opposite expression pattern during stem development.The MYC genes exhibited distinct expression pattern under different hormone treatments and abiotic stress,but most gene pairs shared similar expression patterns.[Conclusion]Different MYC members may be involved in different biological processes.The results of this study provide a reference for further decipher the function of MYCs in poplar.

关 键 词：毛果杨 MYC基因 表达分析 

分 类 号：S718.46[农业科学—林学]