象皮生肌膏对肛瘘术后模型大鼠创面修复及细胞凋亡的影响  

Effects of Xiangpi Shengji Ointment on wound repair and apoptosis in rats after anal fistula operation

作　　者：刘颖 尹园缘 邹巍莹 黄静雯 程扬 宾东华[1] 詹敏[1] LIU Ying;YIN Yuanyuan;ZOU Weiying;HUANG Jingwen;CHENG Yang;BIN Donghua;ZHAN Min(The First Hospital of Hunan University of Chinese Medicine,Changsha,Hunan 410007,China;Hunan University of Chinese Medicine,Changsha,Hunan 410208,China)

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]湖南中医药大学,湖南长沙410208

出　　处：《湖南中医药大学学报》2023年第6期974-981,共8页Journal of Hunan University of Chinese Medicine

基　　金：湖南省临床医疗技术创新引导项目(2021SK51416);湖南省自然科学基金项目(2023JJ60339);湖南省中医药管理局重点课题(C2022019);长沙市自然科学基金项目(Kq2202458);湖南中医药大学校级课题重点项目(2019XJJJ034);湖南中医药大学中西医结合一流学科开放课题重点项目(2020ZXYJH05);湖南中医药大学中西医结合一流学科开放课题一般项目(2020ZXYJH57);湖南中医药大学研究生创新课题一般项目(2022CX140,2022CX161)。

摘　　要：目的探讨象皮生肌膏对肛瘘术后模型大鼠创面凋亡因子胱天蛋白酶(cysteine aspartic acid specific protease,Caspase)的影响。方法36只SD大鼠建立肛瘘模型后,随机选取27只肛瘘大鼠构建肛瘘术后创面模型,随机分为象皮生肌膏组、湿润烧伤膏组、模型组,每组9只,剩余9只肛瘘大鼠为假手术组。象皮生肌膏组、湿润烧伤膏组使用生理盐水冲洗创面后外敷相应药物,模型组仅使用生理盐水冲洗创面,假手术组无特殊处理,每日1次,连续干预10 d。造模后3、5、7、10 d,观察创面愈合情况,计算创面愈合率。药物干预10 d后,从各组取创面组织,HE染色观察创面组织病理学变化,TUNEL法检测创面组织中细胞凋亡数目,免疫组织化学法检测创面组织中Caspase-3、Caspase-7、Caspase-9、Caspase-12蛋白的表达。结果与模型组相比,象皮生肌膏组在干预后3、5、7、10 d时创面愈合率均显著升高(P<0.05,P<0.01),湿润烧伤膏组在干预后5、7、10 d时创面愈合率均显著升高(P<0.05,P<0.01),而象皮生肌膏组创面愈合率在干预后7、10 d均明显高于湿润烧伤膏组(P<0.01)。与假手术组比较,象皮生肌膏组、湿润烧伤膏组、模型组细胞凋亡率均显著升高(P<0.01),创面组织中Caspase-3、Caspase-7、Caspase-9、Caspase-12蛋白表达水平均显著升高(P<0.01);与模型组比较,象皮生肌膏组、湿润烧伤膏组细胞凋亡率均显著降低(P<0.01),Caspase-3、Caspase-7、Caspase-9、Caspase-12蛋白表达水平均显著下降(P<0.01);与湿润烧伤膏组比较,象皮生肌膏组细胞凋亡率均显著降低(P<0.01),Caspase-3、Caspase-7、Caspase-9、Caspase-12蛋白表达水平均显著下降(P<0.01)。结论象皮生肌膏能通过抑制肛瘘术后创面组织细胞凋亡,抑制凋亡相关因子Caspase-3、Caspase-7、Caspase-9、Caspase-12的表达,促进创面愈合。Objective To explore the effects of Xiangpi Shengji Ointment(XPSJO)on cysteine aspartic acid-specific protease(Caspase)in wound surface of model rats after anal fistula operation.Methods After establishing the anal fistula models in 36 SD rats,27 anal fistula rats were randomly selected to construct wound models after anal fistula operation,then they were randomly divided into XPSJO group,Moisture Exposed Burn Ointment(MEBO)group and model group,with 9 rats in each group.The remaining 9 ones were taken as sham operation group.The XPSJO group and MEBO group were applied with corresponding drugs externally after wound irrigation with normal saline.Wound irrigation with normal saline was performed on model group,while there was no special treatment for the rats in sham operation group.All the above interventions were performed once a day,for 10 consecutive days.On the 3rd,5th,7th,and 10th day after modeling,the wound healing was observed and the wound healing rate was calculated.After 10 days of drug intervention,wound tissue were taken from each group.The histopathological changes of wound tissue were observed by HE staining,the number of cell apoptosis in wound tissue was determined by TUNEL method,and the expressions of Caspase-3,Caspase-7,Caspase-9 and Caspase-12 proteins in wound tissue were measured by immunohistochemistry.Results Compared with model group,the wound healing rate of XPSJO group was higher on the 3rd,5th,7th,and 10th day after intervention(P<0.05,P<0.01),and that of MEBO group was significantly higher on the 5th,7th and 10th day after intervention(P<0.05,P<0.01).Moreover,the wound healing rate of XPSJO group was significantly higher than that of the MEBO group on the 7th and 10th day after drug intervention(P<0.01).Compared with sham operation group,the apoptosis rate and expression levels of Caspase-3,Caspase-7,Caspase-9,and Caspase-12 proteins in wound tissue of XPSJO,MEBO and model groups were significant higher(P<0.01,P<0.01).Compared with model group,the apoptosis rate and expression levels

关 键 词：肛瘘 象皮生肌膏 创面 细胞凋亡 胱天蛋白酶 湿润烧伤膏 

分 类 号：R269[医药卫生—中西医结合]