苦参碱对油酸诱导的脂肪变性Chang Liver细胞的影响及机制  

Effect and mechanism of matrine on steatosis Chang Liver cells induced by oleic acid

作　　者：阳丽梅[1] 庄捷[1] 陈芬燕 黄旭慧[1] YANG Limei;ZHUANG Jie;CHEN Fenyan;HUANG Xuhui(Dept.of Pharmacy,Fujian Provincial Hospital/Provincial Clinical Medical College of Fujian Medical University,Fuzhou 350001,China)

机构地区：[1]福建省立医院/福建医科大学省立临床医学院药学部,福州350001

出　　处：《中国药房》2023年第12期1456-1459,共4页China Pharmacy

基　　金：福建省卫生健康科研人才-中青年骨干人才培养项目(No.2019-ZQN-34)。

摘　　要：目的探讨苦参碱对油酸诱导的脂肪变性Chang Liver细胞的改善作用及可能的机制。方法将Chang Liver细胞分为空白组、模型组和苦参碱低、中、高浓度组(0.1、0.5、1.0 mmol/L)。除空白组外,其余各组细胞使用1.0 mmol/L油酸处理24 h建立脂肪变性细胞模型,苦参碱各剂量组加入相应浓度药物干预24 h。检测细胞活性,观察细胞内脂滴形态,测定细胞中的脂质含量,检测细胞中肝功能指标[丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、总胆红素(TBIL)、碱性磷酸酶(ALP)]含量和法尼醇X受体(FXR)、细胞色素P4507A1(CYP7A1)、成纤维细胞生长因子19(FGF19)的mRNA及蛋白表达水平。结果油酸和苦参碱对Chang Liver细胞活性无明显影响。经油酸处理后的细胞内可见橘红色脂滴;与空白组比较,其相对脂质含量、肝功能指标水平均显著升高,FXR、CYP7A1、FGF19 mRNA及蛋白表达水平均显著降低(P<0.05)。经低、中、高浓度苦参碱干预后,上述指标均显著逆转(P<0.05)。结论苦参碱可通过调控FXR/CYP7A1/FGF19信号通路来改善油酸诱导的脂肪变性Chang Liver细胞的脂质含量和肝功能指标。OBJECTIVE To investigate the effects of matrine(MT)on steatosis Chang Liver cell model induced by oleic acid(OA)and its possible mechanism.METHODS Chang Liver cells were divided into blank group,model group and MT low-dose,medium-dose group and high-dose groups(0.1,0.5,1.0 mmol/L).Except for blank group,the other groups were treated with 1.0 mmol/LOA for 24 h to establish steatosis model,and MT groups were given corresponding concentrations of drugs for 24 h.The activities of steatosis Chang Liver cells were observed;the morphologies of intracellular lipid droplets were observed and lipid content was also determined.The contents of liver function indexes[alanine transaminase(ALT),aspartate transaminase(AST),total bilirubin(TBIL),alkaline phosphatase(ALP)],as well as mRNA and protein expressions of farnesoid X receptor(FXR),cytochrome P4507A1(CYP7A1)and fibroblast growth factor 19(FGF19)were all detected.RESULTS OA and MT had no significant effect on the activity of Chang Liver cells.After OA treatment,orange lipid droplets formed in cytoplasm;compared with blank group,relative lipid content and the levels of liver function indexes were increased significantly,while the mRNA and protein expressions of FXR,CYP7A1 and FGF19 were down-regulated significantly(P<0.05).After treatment of low,medium and high concentrations of MT,above indexes were all reversed significantly(P<0.05).CONCLUSIONS MT could significantly improve the lipid content and liver function indexes of steatosis Chang Liver cells induced by OA though regulating FXR/CYP7A1/FGF19 signaling pathway.

关 键 词：苦参碱 Chang Liver细胞 脂代谢 非酒精性脂肪性肝病 

分 类 号：R965[医药卫生—药理学]