Antioxidant and Cytotoxic Activities of A Novel Isomeric Molecule(PF5)Obtained from Methanolic Extract of Pleurotus Florida Mushroom  

作　　者：D.Menaga Pattanathu K.S.M.Rahman S.Rajakumar P.M.Ayyasamy 

机构地区：[1]Department of Microbiology,Periyar University,Salem 636011,India [2]Department of Microbiology,Vivekanandha College of Arts and Sciences for Women,Tiruchengode 637205,India [3]Research and Development,Tara Biologics Limited,Woking,Surrey GU216BP,United Kingdom [4]Department of Marine Biotechnology,Bharathidasan University,Tiruchirappalli 620024,India

出　　处：《Journal of Bioresources and Bioproducts》2021年第4期338-349,共12页生物质资源与工程（英文）

摘　　要：The Pleurotus florida is recognized as a medicinal and edible mushroom and the present study intends to reveal the active isomeric molecules from this mushroom.The P.florida was cultivated using different nutrient supplements:groundnut husk,maize powder,horse gram powder and coconut oil-cake powder.Horse gram supplement showed the higher mushroom yield and henceit was used for the cultivation of P.florida.Methanolic extract of P.florida was found to be efficient in antioxidant activity among ethanol,aqueous,ethyl acetate and hexane extracts.The bioactive fraction 3-methoxy-4-hydroxy cinnamic acid(PF5)was isolated and purified from the methanolic extract of P.florida by column chromatography,thin layer chromatography(TLC)and gas chromatography-mass spectrum(GC-MS)and further it was characterized by Nuclear magnetic resonance(NMR).The PF5 was tested for its DPPH and reducing power assays,and the IC_(50) values were found to be 21.7μg/mL and 105μg/mL,respectively.We found that the cytotoxic effect of 3-methoxy-4-hydroxy cinnamic acid was tested against the lung cancer cell line using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT),sulphorodamine B(SRB)and trypan blue assays which exhibited a higher cytotoxic effect(CTC_(50),645μg/mL).These results suggested that 3-methoxy-4-hydroxy cinnamic acid from P.florida could be explored as a novel and potent natural antioxidant and cancer preventive agent,alternative to existing synthetic molecules.

关 键 词：Pleurotus florida Methanolic extract Isomeric molecule Antioxidant activity Cytotoxic activity 

分 类 号：Q94[生物学—植物学]