Enhancement of Essential Oil Yield from Melaleuca Leucadendra L.Leaves by Lignocellulose Degradation Pre-Treatment Using Filamentous Fungi  

作　　者：Tjokorda I.Indira Khairul Hadi Burhan Robert Manurung Ana Widiana 

机构地区：[1]Department of Bioengineering,School of Life Science and Technology,Institute of Technology Bandung,Indonesia Agriculture and Bioproduct Technology Research Group Institute of Technology Bandung,Jl.Raya Jatinangor Km 20.75,Sumedang 45363,Indonesia [2]Department of Biology,Faculty of Sciences and Technology Sunan Gunung Djati University,Jl.A.H.Nasution No.105,Bandung 40614,Indonesia

出　　处：《Journal of Bioresources and Bioproducts》2021年第4期379-386,共8页生物质资源与工程（英文）

摘　　要：The essential oil from Melaleuca leucadendra L.leaves has been widely used as a perfume and traditional remedy,cosmetics and pharmaceutical products ingredient since many years ago.The common technology to recover the oil is hydro-distillation and steam-distillation.However,all oil can not be fully extracted from the leaves by this method due to the recalcitrant structure of leaves that hindrance the access of the solvent.Adding a submerged fermentation as a pretreatment step prior to the extraction process helped to loosen the lignocellulose structure and enhance oil release in the extraction process.In this study,the raw materials were collected from the natural forest in Buru Island,Maluku,Indonesia.The biological agents applied in these processes were Phanerochaete chrysosporium ITBCC136 and Trichoderma viride ITBCC143.The oil extraction process was conducted by method of steam-distillation,the oil was analysed using gas chromatography-mass spectroscopy(GC-MS),and the lignocellulose content in the biomass was measured by the fractionation method.The treatment using T.viride provided the highest increase in yield percentage up to 3.47%as compared with control of 1.45%,with the lowest percentages of the remained cellulose,while the fermentation with the presence of P.chrysosporium did not affect the oil yield even the lignin content was decrease as much as 21%.The percentages of 1,8-cineole in the oil were almost unchanged,which was about 20%of the oil.

关 键 词：Cajuput oil CINEOLE Filamentous fungi Lignocellulose biodegradation Phanerochaete chrysosporium Trichoderma viride 

分 类 号：TQ645.1[化学工程—精细化工]