食物黄酮异荭草素及其银纳米颗粒的保肝活性  

Hepatoprotective Effect of Isoorientin and Its Silver Nanoparticle

作　　者：王晓 张忠[1] 袁莉[1] WANG Xiao;ZHANG Zhong;YUAN Li(College of Food Engineering and Nutritional Science,Shaanxi Normal University,Xi'an 710119,China)

机构地区：[1]陕西师范大学食品工程与营养科学学院,陕西西安710119

出　　处：《食品与生物技术学报》2023年第5期102-111,共10页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金面上项目(31972183);中央高校基本科研业务费专项资金资助项目(GK202002006);西安市青年人才托举计划项目(095920201311);西部果品资源高值利用教育部工程研究中心开放课题项目(XGZX2021-G10);陕西省重点研发计划项目(2021NY-145);陕西省技术创新引导专项项目(2022QFY09-03)。

摘　　要：为提高食物黄酮异荭草素(Iso)的生物活性和预防苯并(a)芘(BaP)诱发的肝损伤提供一定的依据,以Iso及其银纳米颗粒(Iso-AgNPs)为研究对象,建立食品高温有害产物BaP损伤HL-7702人肝细胞模型,基于细胞凋亡、自噬和焦亡3种程序性死亡方式,通过MTT、台盼蓝染色、荧光标记、流式细胞术、Western blotting等方法检测Iso-AgNPs的保肝作用。结果表明:相较于Iso,Iso-AgNPs能更好地钝化BaP对HL-7702人肝细胞活力的作用,促进细胞增殖,显著抑制BaP诱导的HL-7702人肝细胞凋亡性、自噬性和焦亡性损伤。因此,Iso-AgNPs可提高Iso的生物活性且预防肝细胞损伤。To improve the biological activity of food flavone isoorientin(Iso)and provide a basis for preventing liver injury induced by benzo(a)pyrene(BaP),Iso and its silver nanoparticles(Iso-AgNPs)were used to investigate to the damage of HL-7702 human liver cells caused by BaP,a harmful product of food prepared under high temperature.The hepatoprotective effect of Iso-AgNPs was detected by MTT,trypan blue staining,fluorescence labeling,flow cytometry,and Western blotting based on the three programmed death modes including apoptosis,autophagy and pyroptosis.The results showed that compared with Iso,Iso-AgNPs could better inhibit the damage of HL-7702 huamn liver cells activity induced by BaP,promote cell proliferation,and significantly inhibit the apoptosis,autophagy and apoptosis of HL-7702 huamn liver cells.Therefore,Iso-AgNPs could improve the biological activity of Iso and prevent liver cell damage.

关 键 词：银纳米颗粒负载异荭草素 异荭草素 细胞凋亡 细胞自噬 细胞焦亡 

分 类 号：TQ28[化学工程—有机化工]