Establishment of Characteristic Chromatogram of Honeysuckle Stem Formula Granules Based on the Concept of Whole Process Quality Control  

作　　者：Minling WEI Haixi LI Xiongmei HUANG Bingli LIANG Hongyan WEI 

机构地区：[1]Purapharm(Nanning)Pharmaceuticals Co.,Ltd.,Nanning 530007,China

出　　处：《Medicinal Plant》2023年第3期29-32,37,共5页药用植物：英文版

基　　金：Supported by Major Project of Nanning Scientific Research and Technology Development Plan in 2020(20201048)。

摘　　要：[Objectives]To establish a HPLC characteristic spectrum of honeysuckle stem formula granules,and to evaluate the quality consistency based on the standard decoction.[Methods]Using Agilent ZORBAX SB-C_(18)(5μm,250 mm×4.6 mm)chromatographic column;gradient elution with acetonitrile-0.4%phosphoric acid solution(0.2:99.8,V/V),column temperature at 25℃;detection wavelength at 236 nm;flow rate 1.0 mL/min;using"Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine",to evaluate the similarity of the characteristic chromatogram of crude drug,standard decoction and formula granules of honeysuckle stem.[Results]The similarity of characteristic chromatogram was higher than 0.94,and 6 components were identified from 10 characteristic peaks.[Conclusions]The established method can more comprehensively reflect the overall appearance of the chemical substances in the honeysuckle stem and the transmission law,and can provide an experimental basis for the consistency of the quality of the honeysuckle stem formula granules.

关 键 词：Honeysuckle stem formula granules Standard decoction Characteristic chromatogram HPLC Correlation Quality control 

分 类 号：R283.6[医药卫生—中药学]