磷酸三(2-氯乙基)酯对紫花苜蓿种子萌发及其抗氧化特性的影响  

Effects of tris(2-chloroethyl)phosphate on the seed germination and antioxidant properties of alfalfa

作　　者：张丽佳 杨程[2] 苗宇 王婕 赵尧尧 刘美君 张浩洋 ZHANG Lijia;YANG Cheng;MIAO Yu;WANG Jie;ZHAO Yaoyao;LIU Meijun;ZHANG Haoyang(College of Prataculture, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China;Wheat Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China)

机构地区：[1]新疆农业大学草业学院,新疆乌鲁木齐830052 [2]河南省农业科学院小麦研究所,河南郑州450002

出　　处：《草业科学》2023年第5期1274-1283,共10页Pratacultural Science

基　　金：国家自然科学基金(31860683);财政部和农业农村部:国家现代农业产业技术体系。

摘　　要：磷酸三(2-氯乙基)酯(TCEP)是一种新型阻燃材料,在环境中不断积累,对自然生态和人类健康造成危害。本试验以新疆广泛种植的紫花苜蓿(Medicago sativa)为材料,设置不同浓度TCEP和不同处理时间,研究了TCEP对紫花苜蓿种子萌发特性、活性氧产生和抗氧化系统的影响。结果表明,高浓度的TCEP(≥120 mg·L^(−1))对苜蓿萌发具有显著的抑制效应(P<0.05),且抑制程度随着处理浓度的增加逐渐增大。通过组织化学染色和分光光度法测定发现TCEP处理的苜蓿芽苗体内活性氧剧增细胞膜脂过氧化程度加剧,第3天时芽苗的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)、脱氢抗坏血酸还原酶(DHAR)、单脱氢抗坏血酸还原酶(MDHAR)和谷胱甘肽过氧化物酶(GSH-Px)显著提高,氧化型谷胱甘肽(GSSH)和氧化型抗坏血酸(DHA)含量增加。处理第5时,SOD、CAT、DHAR和MDHAR活性升高,GSSH含量增加。以上结果表明在TCEP胁迫下,苜蓿体内抗坏血酸-谷胱甘肽(AsA-GSH)循环的正常进行受到干扰,还原型抗坏血酸(AsA)和还原型谷胱甘肽(GSH)的氧化程度升高,从而破坏了苜蓿芽苗内的氧化还原平衡,使活性氧大量积累,最终导致苜蓿萌发受到抑制。本研究初步探明了紫花苜蓿对TCEP污染的耐受性以及TCEP对苜蓿萌发的毒害作用及具体机制,对未来可能发生的草地TCEP污染的修复提供了理论依据。Tris(2-chloroethyl)phosphate(TCEP)is a new type of flame-retardant material that continues to accumulate in the environment and causes harm to the natural ecology and human health.In this experiment,the effects of TCEP on the seed germination characteristics,reactive oxygen species production,and antioxidant system were studied using widely cultivated alfalfa as the raw material,with different concentrations of TCEP and different treatment times.The results showed that high concentrations of TCEP(≥120 mg·L^(−1))had a significant inhibitory effect on alfalfa germination,and the degree of inhibition gradually increased with an increase in the treatment concentration.Histochemical staining and UV spectrophotometric determination showed that the degree of lipid peroxidation of the reactive oxygen species in TCEP-treated alfalfa sprouts was exacerbated,and the SOD,CAT,POD,APX,DHAR,MDHAR,and GSH-Px of the sprouts increased significantly on the third day,and the contents of GSSH and DHA also increased.On day 5 of the treatment,the SOD,CAT,DHAR,and MDHAR activities and GSSH content increased.The above results show that under TCEP stress,the normal progress of AsA-GSH circulation in alfalfa is disturbed,and the oxidation degree of AsA and GSH increases,thereby destroying the redox balance in the alfalfa shoots,causing a large accumulation of reactive oxygen species,and finally inhibiting alfalfa germination.This study investigated the tolerance of alfalfa to TCEP pollution and the toxic effects and specific mechanisms of TCEP on alfalfa germination,which will provide a theoretical basis for the restoration of TCEP pollution in grasslands that may occur in the future.

关 键 词：有机磷酸酯 根芽长 生物量 抗坏血酸-谷胱甘肽循环 活性氧 抗氧化系统 组织化学染色 

分 类 号：S541.9[农业科学—作物学]