干旱胁迫下心叶驼绒藜生理响应特性  

Physiological responses of Krascheninnikovia ewersmannia under drought stress

作　　者：柯梅 侯钰荣 魏鹏 兰吉勇[1] 康帅 李超 KE Mei;HOU Yurong;WEI Peng;LAN Jiyong;KANG Shuai;LI Chao(Grassland Research Institute of Xinjiang Academy of Animal Science,Urumqi 830011,Xinjiang,China)

机构地区：[1]新疆维吾尔自治区畜牧科学院草业研究所,新疆乌鲁木齐830011

出　　处：《草业科学》2023年第5期1349-1357,共9页Pratacultural Science

基　　金：自治区公益性项目“五种荒漠植物芽期抗旱性评价”(ZY2022022);自治区基金面上项目(2020D01A38)。

摘　　要：以心叶驼绒藜(Krascheninnikovia ewersmannia)为研究材料,用0(CK)、5%、15%、25%、30%、35%聚乙二醇(PEG-6000)模拟干旱胁迫,从渗透性调节物质、保护性物质、脂肪酸组分等方面研究其对胁迫的生理响应。结果显示,在5%、15%低浓度PEG处理下,驼绒藜幼苗通过增加渗透调节物质脯氨酸含量、启动抗氧化酶系统、保持膜脂中的饱和脂肪酸和不饱和脂肪酸动态平衡来减弱膜脂过氧化对细胞膜系统的损害;在25%PEG处理下,丙二醛含量急剧增加,叶片膜脂过氧化程度加重,植物通过大幅提高脯氨酸含量、增强抗氧化酶活性、增大膜脂中不饱和脂肪酸含量减少干旱胁迫带来的膜脂损伤;在30%、35%高浓度PEG处理下,脯氨酸含量下降、抗氧化酶系统活性降低、饱和脂肪酸含量增加,膜脂受损害严重。由此得出,驼绒藜幼苗在PEG处理液为25%时,渗透调节物质、保护性物质、不饱和脂肪酸等物质协同作用形成高效的防御系统以增强对干旱胁迫的抗性。The physiological responses of Krascheninnikovia ewersmannia to drought stress was studied using 0(CK),5%,15%,25%,30%,and 35%polyethylene glycol(PEG-6000)to simulate drought stress from the aspects of osmotic regulation substances,protective substances,and fatty acid components.The results showed that under the 5%,15%low concentration of PEG treatment,the damage of membrane lipid peroxidation to the cellular membrane system was weakened through increasing the content of osmotic adjustment substance proline,initiating the antioxidant enzyme system,and maintaining the dynamic balance of saturated fatty acids and unsaturated fatty acids in membrane lipids;under the treatment of 25%PEG,the content of malondialdehyde increased sharply and the degree of membrane lipid peroxidation was aggravated.Plants can reduce membrane lipid damage caused by drought stress by significantly increasing the proline content,enhancing antioxidant enzyme activity,and increasing unsaturated fatty acid content in membrane lipid;under the 30%,35%high concentration PEG treatment,proline content decreased,antioxidant enzyme system activity decreased,saturated fatty acid content increased,and membrane lipids were seriously damaged.It is concluded that with the 25%PEG treatment solution,osmoregulatory substances,protective substances,unsaturated fatty acids,and other substances act together to form an efficient defense system to enhance the resistance to drought stress.

关 键 词：心叶驼绒藜 PEG胁迫 渗透调节 抗氧化酶 脂肪酸 抗旱性 

分 类 号：Q945.78[生物学—植物学]