乳酸菌改善苜蓿、无芒雀麦混贮发酵品质及CNCPS蛋白组分  

Effects of additives on fermentation quality and CNCPS protein fractions of legume–grass silage mixture

作　　者：赵璐洁 范华芳 孙鑫畅 张霞[1] 腾美美 高诚泽 颜安[1] 谢开云 万江春[1] ZHAO Lujie;FAN Huafang;SUN Xinchang;ZHANG Xia;TENG Meimei;GAO Chengze;YAN An;XIE Kaiyun;WAN Jiangchun(College of Grassland Science,Xinjiang Agricultural University/Xinjiang Key Laboratory of Grassland Resources and Ecology,Urumqi 830052,Xinjiang,China)

机构地区：[1]新疆农业大学草业学院/新疆草地资源与生态重点实验室,新疆乌鲁木齐830052

出　　处：《草业科学》2023年第5期1397-1409,共13页Pratacultural Science

基　　金：新疆维吾尔自治区自然科学基金(2022D01A69);新疆维吾尔自治区重点研发任务专项计划:优质牧草高效生产与加工关键技术集成示范(2022B02003)。

摘　　要：为探究添加剂对不同混播比例混贮饲料品质以及蛋白组分的影响。试验以豆禾1꞉2和豆禾2꞉2的紫花苜蓿(Medicago sativa)和无芒雀麦(Bromus inermis)混播牧草为原料,分别设置对照和1×10^(5) cfu·g^(−1)的植物乳杆菌(Lactobacillus plantarum)和干酪乳杆菌(Lactobacillus casei)处理,在青贮的0、1、3、7、15、30和60 d动态测定混贮饲料的蛋白组分和蛋白酶活性,并于青贮60 d后测定混贮饲料的发酵品质、营养成分和氮组分。结果表明:添加剂可降低豆禾混贮饲料中的非蛋白氮和结合蛋白,降低羧基肽酶活性,同时可降低混贮饲料的pH和氨态氮,并增加乳酸,起到改善豆禾混贮饲料品质的作用。豆禾1꞉2为混贮原料制成的混贮饲料品质优于豆禾2꞉2;添加植物乳杆菌后的豆禾1:2混贮饲料,其pH 3.74,乳酸含量为85.3 g·kg^(−1),V-Score评分为90,发酵品质最好。Medicago sativa and Bromus inermis with ratios of 1꞉2 and 2꞉2 legume–grass mixtures were used as material to study the effects of additives on fermentation quality and CNCPS protein fractions of mixed forage with different mixed sowing proportion.The control,Lactobacillus plantarum and Lactobacillus casei treatment were set,respectively.The LAB inoculant were applied at the rate of 1.0×10^(5) cfu·g^(−1).The protein components and protease activities of the mixed forage were dynamically measured at 0,1,3,7,15,30 and 60 d after silage.The fermentation quality,nutrient components,and nitrogen components were measured at 60 d after silage.The results have shown that the additives could reduce the non protein nitrogen,conjugated protein,carboxypeptidases activity,pH,and ammonia nitrogen.In contrast,it increased the lactic acid,therefore,improving the quality of the legume–grass silage mixture.The quality of the ratio of 1꞉2 legume–grass silage mixture was higher than that of 2꞉2 legume–grass silage mixtures.The ratio of 1꞉2 legume–grass silage mixture with Lactobacillus plantarum added had the highest fermentation quality with pH 3.74,lactic acid content of 85.3 g·kg^(−1),and a DM and V-Score of 90.

关 键 词：紫花苜蓿 无芒雀麦 植物乳杆菌 干酪乳杆菌 蛋白酶 豆禾混播草地 青贮品质 

分 类 号：S816.53[农业科学—饲料科学]