牛尾独活叶抗红色毛癣菌活性成分研究  

Anti-Trichophyton rubrum Active Ingredients in Leaves of Heracleum vicinum Boiss.

作　　者：吴海顺 刘谋焱 于华忠[1] 陈汇新 WU Haishun;LIU Mouyan;YU Huazhong;CHEN Huixin(Hunan Key Laboratory of Forest Chemical Engineering,Jishou University,Zhangjiajie 427000,China;Guangdong Yutong Pharmaceutical Biotechnology Co.,Ltd.,Dongguan 523000,China)

机构地区：[1]吉首大学林产化工工程湖南省重点实验室,湖南张家界427000 [2]广东宇通医药生物科技有限公司,广东东莞523000

出　　处：《林产化学与工业》2023年第3期130-136,共7页Chemistry and Industry of Forest Products

摘　　要：为了解牛尾独活叶的抗红色毛癣菌活性,以抑菌圈大小为考察指标,对比了牛尾独活不同部位、其它独活品种和常见的抗红色毛癣菌植物之间的差异,通过单因素试验和响应面法优化牛尾独活叶提取工艺,并对牛尾独活叶提取液进行溶剂萃取和化学成分预试分析。研究结果表明:伞形科不同属植物的抗红色毛癣菌活性具有差异性,牛尾独活具有较好的抑菌活性,其叶、种子、茎和根的抑菌活性差异不大。牛尾独活叶最佳抗红色毛癣菌活性的提取工艺为:乙醇体积分数25%、液料比15∶1(mL∶g)、超声波功率220 W、提取温度80℃、提取时间30 min,在此工艺条件下,牛尾独活叶提取液对红色毛癣菌的抑菌圈为(22±0.2)mm;牛尾独活叶提取液中抑菌成分均能较好地溶解在石油醚、乙酸乙酯和正丁醇中,其中,石油醚萃取液抑菌效果最好;化学成分预试显示牛尾独活叶具有抗红色毛癣菌的活性成分可能是多酚类和黄酮类化合物。In order to understand the anti-Trichophyton rubrum activity of the leaves of Heracleum vicinum,the difference of anti-T.rubrum activity between different parts of H.vicinum,other varieties of H.vicinum and some plants with anti-T.rubrum activity were studied using the size of inhibition zone as the index.The extraction process of H.vicinum leaves was optimized by single factor test and response surface method,and the solvent extraction and chemical composition pre-test analysis of H.vicinum leaves extract were carried out.The results showed that the anti-T.rubrum activities of different genera of Umbelliferae were different.H.vicinum had good antibacterial activity,and the antibacterial activities of leaves,seeds and roots were not significantly different.The optimal extraction conditions for the anti-T.rubrum activity of the leaves of H.vicinum was as follows:ethanol volume fraction of 25%,liquid-solid ratio of 15∶1(mL∶g),ultrasonic power of 220 W,extraction temperature of 80℃,and extraction time of 30 min.Under these process conditions,the inhibition zone of the extract of H.vicinum leaves against T.rubrum was(22±0.2)mm.The antibacterial components in the extract of H.vicinum leaves could be well dissolved in petroleum ether,ethyl acetate and n-butanol,where the petroleum ether extract had the best antibacterial effect.The preliminary test of chemical components showed that the active components of the leaves of H.vicinum against T.rubrum might be phenolic and flavonoid active components.

关 键 词：牛尾独活叶 皮肤癣病 红色毛癣菌 提取 化学成分预试 

分 类 号：TQ35[化学工程] R756.3[医药卫生—皮肤病学与性病学]