HCMV-DNA在不同类型标本中的检出率差异研究  

Study on the difference of detection rate of HCMV-DNA in different types of specimens

作　　者：徐爽 吕晓丽 王琳 郜赵伟 董轲 XU Shuang;LV Xiaoli;WANG Lin;GAO Zhaowei;DONG Ke(Department of Clinical Laboratory,the Second Affiliated Hospital of Air Force Military Medical University,Xi′an,Shaanxi 710038,China)

机构地区：[1]空军军医大学第二附属医院检验科,陕西西安710038

出　　处：《国际检验医学杂志》2023年第S01期123-126,共4页International Journal of Laboratory Medicine

摘　　要：目的分析不同标本种类检测不同疾病患者人巨细胞病毒核酸(HCMV-DNA)的阳性率,为临床患者进行HCMV感染诊治提供依据。方法以该院2013年至2021年接诊患者(住院、门诊)为分析对象,采用实时荧光定量PCR法对患者血清、尿液、乳汁(幼儿患者母亲乳汁)、组织(肠黏膜病变组织)进行HCMV-DNA检测。结果不同疾病患者,标本种类不同,检出率大相径庭,血液病患者血清检测HCMV-DNA的阳性率为17.86%,而尿液的阳性率为63.10%,差异有统计学意义(χ^(2)=36.675,P<0.001);溃疡性结肠炎(UC)患者血清检测HCMV-DNA的阳性率为4.16%,而组织的阳性率为79.17%,差异有统计学意义(χ^(2)=55.543,P<0.001);小儿患者血清检测HCMV-DNA的阳性率为8.87%,而小儿尿液阳性率为63.71%,差异有统计学意义(χ^(2)=80.644,P<0.001),小儿患者血清检测HCMV-DNA的阳性率为9.09%,而小儿其母亲乳汁的阳性率为74.54%,差异有统计学意义(χ^(2)=32.825,P<0.001)。结论血液病患者选择尿液检测HCMV-DNA比血清检出率高,溃疡性结肠炎(UC)患者选择肠黏膜病变组织检测HCMV-DNA比血清检出率高,小儿患者可联合检测尿液及其母亲乳汁HCMV-DNA,为小儿感染HCMV诊断提供了有力的依据;故标本种类的选择对患者HCMV感染的早期诊断有重要意义。Objective To analyze the positive rate of HCMV-DNA in patients with different diseases,and to provide evidence for diagnosis and treatment of HCMV infection in clinical patients.Methods The patients(inpatients and outpatients)admitted to the Second Affiliated Hospital of Air Force Military Medical University from 2013 to 2021 were analyzed.The serum,urine,Milk(mother′s milk of infant patients)and Tissue(mucosal lesion tissue)of the patients were detected by real-time fluorescence quantitative PCR.Results The positive rate of HCMV-DNA was17.86%in serum and 63.10%in urine,the difference was statistically significant(χ^(2)=36.675,P<0.001).The positive rate of HCMV-DNA was 4.16%in serum and79.17%in tissue of patients with ulcerative colitis(UC),the difference was statistically significant(χ^(2)=55.543,P<0.001).The positive rate of serum HCMV-DNA in children was 8.87%,The positive rate of urine in children was 63.71%,the difference was statistically significant(χ^(2)=80.644,P<0.001).The positive rate of HCMV-DNA in serum of children was 9.09%.The positive rate of children′s mother′s milk was 74.54%,the difference was statistically significant(χ^(2)=32.825,P<0.001).Conclusion The detection rate of HCMV-DNA in urine is higher than that in serum in patients with blood diseases,and the detection rate of HCMV-DNA in intestinal mucosal lesions is higher than that in serum in patients with UC.The combined detection of HCMV-DNA in urine and mother′s milk in children provides a strong basis for the diagnosis of HCMV infection in children.Therefore,the selection of specimens is of great significance for the early diagnosis of HCMV infection in patients.

关 键 词：尿液 乳汁 组织 血液病 人巨细胞病毒核酸 

分 类 号：R446[医药卫生—诊断学]