白细胞介素-35和辅助性T淋巴细胞17/调节性T淋巴细胞在正常妊娠期母体外周血中的表达  

Expression level of IL-35 and TH17/Treg cells in maternal serum during pregnancy

作　　者：胡学亭 魏红[1] 李玉娟[1] 刘义帅[1] 庄文玉 于北凯 HU Xue-ting;WEI Hong;LI Yu-juan;LIU Yi-shuai;ZHUANC Wen-yu;YU Bei-kai(Department of Clinical Laboratory Weifang Traditongal Chinese Medical Hospital,Weifang,Shandong 261041,China)

机构地区：[1]潍坊市中医院检验科,山东潍坊261041

出　　处：《中国妇幼保健》2023年第11期2076-2080,共5页Maternal and Child Health Care of China

基　　金：山东省潍市卫生计生委计划项目(2017wsjs109)。

摘　　要：目的检测抑制性细胞因子白细胞介素-35(IL-35)、调节性T淋巴细胞(Treg)及辅助性T淋巴细胞17(Th17)在女性妊娠期、非妊娠期及不明原因复发性自然流产(URSA)患者中的表达水平,探讨IL-35在维持妊娠母胎免疫耐受中的作用。方法选择2018年3月—2019年9月就诊于潍坊市中医院妇产科门诊的既往无流产史的正常健康妊娠女性90例,不明原因复发性自然流产患者30例,选取同一时期的非妊娠期育龄健康女性30例,按照妊娠周数将妊娠期分为3组,其中妊娠早期组30例、妊娠中期组30例及妊娠晚期组30例。采用酶联免疫吸附试验测定其外周血中IL-35、IL-17的表达水平;流式细胞技术测定辅助性T细胞17(Th17)、调节性T细胞(Treg)的表达,并计算Th17/Treg比值。结果妊娠早期、妊娠中期及妊娠晚期女性IL-35表达均显著高于非妊娠女性和URSA患者(均P<0.01),妊娠各期IL-17显著低于复发性自然流产患者(P<0.01);与复发性自然流产比较,妊娠期各组女性Th17细胞比例表达明显降低(均P<0.01),Treg细胞比例表达明显升高(均P<0.01),Th17/Treg细胞比值显著下降(均P<0.01);与非妊娠女性比较,IL-17、Th17细胞比例、Treg细胞比例及Th17/Treg细值比例表达差异无统计学意义(P>0.05)。结论IL-35在健康妊娠女性中表达增加,Th17/Treg细胞比值降低有利于维持妊娠期母胎免疫耐受,二者可能共同参与了妊娠早期母胎免疫耐受的的建立与维持。Objective To detect the expression level of IL-35,Treg and Th17 cells in women with or without pregnant and recurrent spontaneous abortion(URSA),and to explore the role of IL-35 in maintenance of maternal-fetal immune tolerance.Methods from March 2018 to September 2019,90 normal pregnant women with no previous abortion history,30 patients with unxxplained recurrent spontaneous abortion were enrolled in this study,and 30 healthy non-pregnant women of childrenbearing age at the same period were selected.According to the gestational weeks,the gregnant women were divided into three groups:30 cases in early pregnancy group,30 cases in middle pregnancy group and 30 cases in late pregnancy group.Level of IL-35,IL-17 in maternal serum were analyzed by enzyme-linked immunosorbent assay(ELISA),expression of helper T cells(Th17)and regulatory T cells(Treg)was analyzed by flow cytometry,and the Th17/Treg ratou was calculated.Results Expression level of IL-35 in early,middle and late pregnancy wass significantly higher than that in nonpregnant women and patients with URSA(P<0.01);Compared with recurrent spontaneous abortion,the proportion of TH17 cells was significantly decreased(P<0.01),proportion of Treg cells was significantly increased(P<0.01),and the ratio of Th17/Treg cells was significantly decreased(P<0.01);Compared with nopregnant women,xpression level of IL-17,Th17,Treg and Th17/Treg was not significant different(P>0.05).Conclusion The expression level of IL-35 increased in healthy pregnant women and decrease of Th17/Treg ratio was benefical to the maintence of maternal-fetal immune tolerance during pregnancy.

关 键 词：妊娠免疫耐受 白细胞介素-35 白细胞介素-17 调节性T淋巴细胞 辅助性T淋巴细胞17 

分 类 号：R714.1[医药卫生—妇产科学]