5-氮杂胞苷对变应性鼻炎大鼠DNA甲基转移酶1基因调节作用的机制研究  

Study on the mechanism of 5-azacytidine on DNA methyltransferase 1 gene in allergic rhinitis rats

作　　者：贾宏林 张茹 卜倩[3] 史红[2] 姜孝芳 JIA Hong-lin;ZHANG Ru;BO Qian;SHI Hong;JIANG Xiao-fang(The Fourth Clinical School of Medicine,Xinjiang Medical University,Urumqi 830000,Xinjiang Province,China;College of Traditional Chinese Medicine,Xinjiang Medical University,Urumqi 830000,Xinjiang Province,China;Affiliated Hospital of Traditional Chinese Medicine of Xinjiang Medical University,Urumqi 830000,Xinjiang Province,China;Shenzhen Hypross Future Medical Laboratory,Shenzhen 518000,Guangdong Province,China)

机构地区：[1]新疆医科大学第四临床医学院,新疆乌鲁木齐830000 [2]新疆医科大学中医学院,新疆乌鲁木齐830000 [3]新疆医科大学附属中医医院眼科,新疆乌鲁木齐830000 [4]深圳海普洛斯未来医学检验实验室,广东深圳518000

出　　处：《中国临床药理学杂志》2023年第10期1466-1469,共4页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(81760182);深圳市科技研发基金资助项目(JSGG20180508152646606);深圳市科技研发基金资助项目(KQJSCX20180330124428928)。

摘　　要：目的探究甲基化酶抑制药5-氮杂胞苷(5-aza)对DNA甲基转移酶1(DNMT1)的调控作用。方法将大鼠分为正常组、模型组和实验组。正常组大鼠不做干预;模型组大鼠通过腹腔注射卵清蛋白(OVA)和Al(OH)3及OVA局部致敏方法建立变应性鼻炎(AR)模型;实验组大鼠建模后用5 mg·kg^(-1)5-aza进行干预处理。用酶联免疫吸附试验检测大鼠血清免疫球蛋白E(IgE)表达水平;用实时荧光定量聚合酶链反应法检测大鼠DNMT1 mRNA表达情况;用蛋白质印迹法检测大鼠DNMT1蛋白表达情况。结果正常组、模型组和实验组大鼠血清IgE表达水平分别为0.066±0.002、0.074±0.002和0.073±0.003;DNMT1 mRNA的表达水平分别为1.00±0.07、3.92±0.82和0.55±0.16;DNMT1蛋白表达水平分别为0.77±0.13、1.16±0.22和0.40±0.10。模型组的上述指标与正常组比较,差异均有统计学意义(均P<0.01);实验组的上述指标与模型组比较,差异均有统计学意义(均P<0.01)。结论5-aza作为DNA甲基化转移酶抑制药可以通过调控DNMT1表达参与AR大鼠的发生发展。Objective To explore the regulation of methylase inhibitor 5-azacytidine(5-aza)on DNA methyltransferase 1(DNMT1).Methods The rats were divided into normal group,model group and experimental group.The rats in the normal group were not intervened;the model group were injected intraperitoneally with ovalbumin(OVA)and Al(OH)3 and sensitized locally with OVA,allergic rhinitis(AR)rat models were established;after modeling,rats in experimental group were treated with 5 mg·kg^(-1)5-aza intervention.The level of serum immunoglobulin E(IgE)expression was detected by enzyme linked immunosorbent assay;the expression of DNMT1 mRNA was detected by real time quantitative polymerase chain reaction;the expression of DNMT1 protein was detected by Western blot.Results The expression levels of serum IgE in normal group,model group and experimental group were 0.066±0.002,0.074±0.002 and 0.073±0.003,respectively;the expression levels of DNMT1 mRNA in normal group,model group and experimental group were 1.00±0.07,3.92±0.82 and 0.55±0.16,respectively;the expression levels of DNMT1 protein were 0.77±0.13,1.16±0.22 and 0.40±0.10,respectively.The above indexes in model group were significantly different from those in normal group(all P<0.01);the above indexes in experimental group were significantly different from those in model group(all P<0.01).Conclusion As an inhibitor of DNA methyltransferase,5-aza can participate in the occurrence and development of AR rats by regulating the expression of DNMT1.

关 键 词：5-氮杂胞苷 甲基化酶抑制药 变应性鼻炎 DNA甲基化 DNA甲基转移酶1 

分 类 号：R97[医药卫生—药品]