贵州省2017-2019年H3N2亚型流感病毒HA基因遗传特征分析  

Genetic variation characteristics of HA gene of influenza A virus (H3N2) in Guizhou province from 2017 to 2019

作　　者：郑勤妮[1] 任丽娟[1] 庄丽[1] 付成忠 万永虎[1] Zheng Qinni;Ren Lijuan;Zhuang Li;Fu Chengzhong;Wan Yonghu(Department of Virology,Experimental Center,Guizhou Center for Disease Control and Prevention,Guiyang 550004,Guizhou Province,China)

机构地区：[1]贵州省疾病预防控制中心实验中心病毒科,贵阳550004

出　　处：《中国基层医药》2023年第5期692-698,共7页Chinese Journal of Primary Medicine and Pharmacy

基　　金：贵州省科技计划基金(黔科合基础[2020]1Y360);贵州省卫生和计划生育委员会科研基金(gzwjkj2017-1-052)。

摘　　要：目的分析贵州省2017-2019年H3N2亚型流感病毒HA基因特征,了解变异规律。方法由贵州省10家网络实验室分离的H3N2亚型流感毒株中随机选取20株提取RNA,进行逆转录酶聚合酶链反应和测序,采用生物信息软件分析。结果20株毒株HA基因核苷酸同源性为97.7%~100.0%,与世界卫生组织推荐的疫苗株A/Hong-Kong/4801/2014(2017年)和A/Singapore-INFIMH/16-0019/2016(2018年)同源性高,而与2019年世界卫生组织推荐的疫苗株A/Kansas/14/2017(2019年)差异较大。遗传分析显示,20株病毒株主要分为2个分支,2019年流行的毒株处于不同的分支,遗传差距较大。关键位点分析显示,抗原决定族A、B、C和E存在突变;受体结合位点前壁和后壁存在突变;与当年度疫苗株相比,存在糖基化位点的增加。2017-2018年毒株在遗传距离、抗原位点和糖基化位点变异情况与2019年毒株略有不同。结论贵州省2017-2019年H3N2亚型流感病毒HA基因发生基因突变,2019年的毒株变异最为严重,应密切监测H3N2亚型流感病毒遗传进化的变化情况。Objective To analyze the genetic variation characteristics of the HA gene of influenza A virus(H3N2)in Guizhou province from 2017 to 2019.Methods Twenty strains of influenza A virus(H3N2)were randomly selected from 10 network laboratories in Guizhou province for RNA extraction.Reverse transcriptase-polymerase chain reaction and sequencing were performed.The products were analyzed using bioinformatics software.Results The nucleotide homology of the HA gene of the 20 strains was 97.7%-100%,which was highly homologous to the vaccine strains A/Hong-Kong/4801/2014 recommended by WHO in 2017 and A/Singapore-INFIMH/16-0019/2016 recommended by WHO in 2018,but they were significantly different from the vaccine strain A/Kansas/14/2017 recommended by WHO in 2019.Genetic analysis showed that the 20 strains were divided into two branches,and the strains that were prevalent in 2019 were located in different branches,with marked genetic differences.Key site analysis showed mutations in antigenic determinants A,B,C,and E and mutations in the anterior and posterior walls of receptor binding sites.Key site analysis also showed that there was an increase in the number of glycosylation sites compared with the vaccine strains prevalent in the same year.Genetic distance,antigen sites,and glycosylation sites were slightly different between virus strains prevalent in 2017-2018 and virus strains prevalent in 2019.Conclusion The HA gene of the influenza A virus subtype H3N2 in Guizhou province from 2017 to 2019 showed heterogeneity and gene mutation,especially in 2019.Therefore,close monitoring of the genetic evolution of the influenza A virus subtype H3N2 is necessary.

关 键 词：流感病毒A型 H3N2亚型 RNA 病毒 逆转录聚合酶链反应 多位点测序分型 序列同源性 核酸 遗传 序列标记位点 血凝素类 病毒 

分 类 号：R373.13[医药卫生—病原生物学]