养心通脉方对心肌缺血再灌注损伤血瘀证大鼠心肌损伤的治疗作用及其机制  

Therapeutic effect of Yangxin Tongmai formula on myocardial injury in rats with MIRI and blood stasis syndrome

作　　者：黄俊丽 廖琳洁 郑景辉[2] HUANG Junli;LIAO Linjie;ZHENG Jinghui(Graduate School,Guangxi University of Chinese Medicine,Nanning 530299,China)

机构地区：[1]广西中医药大学研究生院,南宁530299 [2]广西中医药大学附属瑞康医院中医科

出　　处：《山东医药》2023年第17期21-25,共5页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(82160875);广西中医药大学“岐黄工程”高层次人才团队培育项目(2021005)。

摘　　要：目的探讨养心通脉方(YTF)对心肌缺血再灌注损伤(MIRI)血瘀证大鼠心肌损伤的治疗作用并分析其相关机制。方法Wistar大鼠分为正常对照组、模型对照组、阴性对照组、阳性对照组、YTF低剂量组、YTF中剂量组、YTF高剂量组,每组10只。除正常对照组外均建立大鼠MIRI血瘀证模型,YTF低剂量组、YTF中剂量组、YTF高剂量组分别给予4.725、9.450、18.900 g/kg YTF灌胃,阳性对照组给予10.710 g/kg冠心苏合丸混悬液灌胃,阴性对照组给予等体积生理盐水灌胃,每日2次,连续给药14 d;正常对照组常规饲养不灌胃。透射电镜观察各组心肌组织及线粒体超微结构,TUNEL荧光染色观察各组心肌细胞凋亡情况,酶标仪检测各组心肌细胞线粒体膜肿胀度(OD540)及线粒体超氧阴离子含量。结果透射电镜观察结果显示,正常对照组心肌肌丝排列整齐,肌节规则,结构清晰,线粒体外膜完整,嵴结构正常,排列致密;模型对照组、阴性对照组心肌肌节断裂,核周间隙增宽,线粒体排列紊乱,部分外膜模糊甚至破裂变形,线粒体嵴结构部分缺失,出现少量自噬体包裹线粒体及细胞质,有少量溶酶体出现;阳性对照组及YTF中、高剂量组线粒体排列较致密,肌丝断裂情况轻,肌节整齐,线粒体外膜及嵴结构较为清晰,可见早期小自噬体数量增多,溶酶体及被自噬体包裹的线粒体增多;YTF低剂量组线粒体肌丝较为完整但肌节不清,线粒体外膜及嵴结构模糊不清,早期小自噬体数量上升。模型对照组、阴性对照组心肌细胞凋亡率高于正常对照组,阳性对照组、YTF中剂量组心肌细胞凋亡率低于阴性对照组(P均<0.05)。模型对照组、阴性对照组OD540低于正常对照组,阳性对照组与YTF低剂量组、YTF中剂量组、YTF高剂量组OD540高于阴性对照组(P均<0.05)。模型对照组、阴性对照组心肌组织超氧阴离子含量高于正常对照组,YTF高剂量组心肌组织超氧�Objective To investigate the therapeutic effect of Yangxin Tongmai formula(YTF)on myocardial injury in rats with myocardial ischemia-reperfusion injury(MIRI)and blood stasis syndrome,and to analyze its related mechanism.Methods Wistar rats were divided into the normal control group,model control group,negative control group,positive control group,low-dose YTF group,medium-dose YTF group and high-dose YTF group,with 10 rats in each group.The rats in the low-dose,medium-dose,and high-dose YTF groups were given 4.725,9.450,and 18.900 g/kg of YTF by gavage,respectively.Rats in the positive control group were given Guanxin Suhe pill suspension at a dose of 10.710 g/kg.Rats in the negative control group were given equal volume of normal saline by gavage,twice a day,for 14 days.Rats in the normal control group were fed routinely without gavage.The apoptosis of cardiomyocytes was observed by TUNEL fluorescence staining.The swelling degree of mitochondrial membrane(OD540)and the content of mitochondrial superoxide anion were detected by microplate labeling instrument.Results The results of transmission electron microscopy showed that in the normal control group,the myocardial myofilaments were arranged neatly,the sarcomere was regular,the structure was clear,the mitochondrial outer membrane was intact,and the cricrists were normal and densely arranged.In the model control group and the negative control group,the myocardial sarcomere was ruptured,the perinuclear space was widened,the mitochondria were arranged disorderly,some outer membrane was blurred or even ruptured and deformed,the mitochondrial crest structure was partially missing,a small number of autophagosomes-wrapped mitochondria and cytoplasm,and a small number of lysosomes appeared.In the positive control group and the medium-dose and highdose YTF groups,the mitochondria were densely arranged,the breakage of myofilaments was light,the sarcomere was neat,the outer membrane and cristulation structure of mitochondria were clear,and the number of small autophagosom

关 键 词：养心通脉方 线粒体 自噬 细胞凋亡 心肌缺血再灌注损伤 血瘀证 

分 类 号：R256.22[医药卫生—中医内科学]