基于GEO数据库的银屑病lncRNA筛选与ceRNA网络构建  

lncRNA screening and ceRNA network construction in psoriasis based on GEO databases

作　　者：范晓美 栗铭钊 牛牧田 高进涛[1,2] FAN Xiaomei;LI Mingzhao;NIU Mutian;GAO Jintao(College of Intelligent Medicine and Biotechnology,Guilin Medical University,Guilin 541199,China;Key Laboratory of Biochemi and Molecular Biology,Guilin Medical University,Guilin 541199,China)

机构地区：[1]桂林医学院智能医学与生物技术学院,广西桂林541199 [2]桂林医学院生物化学与分子生物学重点实验室,广西桂林541199

出　　处：《华夏医学》2023年第2期85-92,共8页Acta Medicinae Sinica

基　　金：国家自然科学基金项目(31860314);广西自然科学基金项目(2018GXNSFAA28104,2016GXNSFBA380146);桂林医学院硕士研究生科研项目(GYYK2021003)。

摘　　要：目的:构建银屑病竞争性内源性RNA(ceRNA)调控网络,为银屑病发病机制提供生物信息学参考。方法:基于基因表达综合数据库(GEO)筛选差异表达的lncRNA并通过相关性分析找到与其相关的编码基因,进行京都基因与基因组百科全书(KEGG)通路富集分析。使用lncBase数据库预测lncRNA的靶向微小RNA(microRNA,miRNA);使用TargetScan数据库预测编码基因靶向miRNA;利用Cytoscape构建ceRNA网络。结果:本研究基于差异表达的lncRNA构建出6个lncRNA(LIN01094、LINC01215、KLF-AS1、TINCR、EMX2OS、C14orf132)介导的lncRNA-miRNA-mRNA ceRNA网络,显示这些lncRNA可能通过影响细胞周期、蛋白酶体、DNA复制、细胞因子-细胞因子受体的相互作用、FoxO信号通路等发挥作用。结论:差异表达的(lncRNA LIN01094、LINC01215、KLF-AS1、TINCR、EMX2OS、C14orf132)可能作为ceRNA发挥调控功能,参与银屑病发病。Objective:To construct a competing endogenous RNA(ceRNA)regulatory network in psoriasis and provide bioinformatics references for the pathogenesis of psoriasis.Methods:The differentially expressed lncRNA were screened based on Gene Expression Omnibus(GEO)database,and their related coding genes were found through correlation analysis.And the enrichment analysis was carried out by using Kyoto Encyclopedia of Genes and Genomes(KEGG).The lncBase database was used to predict the targeting microRNA(miRNA)of lncRNA,and the TargetScan database was used to predict the genes sponging miRNA.And Cytoscape was used to construct ceRNA network.Results:The lncRNA-miRNA-mRNA ceRNA network mediated by 6 lncRNA(LIN01094,LINC01215,KLF-AS1,TINCR,EMX2OS and C14orf132)were constructed based on differentially expressed lncRNA in this study,suggesting these lncRNA might be involved in regulating cell cycle,proteasome,viral protein and cytokine,the interactions of cytokine receptors,and FoxO signaling pathway.Conclusion:The differentially expressed lncRNA(LIN01094,LINC01215,KLF-AS1,TINCR,EMX2OS and C14orf132)may play a regulatory role as ceRNAs,and participate in the pathogenesis of psoriasis.

关 键 词：银屑病 长链非编码RNA 竞争性内源RNA网络 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]