蒙药制剂亚玛丸中羟基红花黄色素A的含量测定方法研究  

Study on Determination of Hydroxysafflower Yellow A in Yama Pill

作　　者：郭司群 迟晓雪 白雅静 GUO Siqun;CHI Xiaoxue;BAI Yajing(Hulunbeier Institute for Food and Drug Control,Hulunbeier 021008,China;Hulunbuir Institute of Forestry and Grassland Science,Hulunbeier 021008,China)

机构地区：[1]呼伦贝尔市食品药品检验所,内蒙古呼伦贝尔021008 [2]呼伦贝尔市林业和草原科学研究所,内蒙古呼伦贝尔021008

出　　处：《中国民族民间医药》2023年第12期30-32,72,共4页Chinese Journal of Ethnomedicine and Ethnopharmacy

摘　　要：目的:建立亚玛丸中羟基红花黄色素A的含量测定方法。方法:采用高效液相色谱法,色谱柱为SHIMADZU-GL WondaCract ODS-2 C_(18)(4.6 mm×250 mm,5μm);流动相为甲醇、乙腈和0.7%磷酸溶液进行洗脱,流速为0.8 mL/min;柱温:35℃;检测波长403 nm。结果:羟基红花黄色素A在2.01~30.15μg/mL的浓度范围内线性关系良好,r=0.9999,回收率为96.5%。结论:该方法经方法学验证准确可行,可用于亚玛丸的质量控制。Objective To establish a method for the determination of Hydroxysafflower yellow A in Yama pill.Methods high performance liquid chromatography was used.The column was SHIMADZU-GL WondaCract ODS-2 C_(18)(4.6 mm×250 mm,5μm),the mobile phase was methanol acetonitrile 0.7%phosphoric acid solution,the flow rate was 0.8 mL/min,the column temperature was 35℃,and the detection wavelength was 403 nm.Results the linear relationship of Hydroxysafflower yellow A was good in the range of 2.01-30.15μg/mL,r=0.9999,the recovery rate was 96.5%.Conclusion The method is proved to be accurate and feasible by methodology,and can be used for the quality control of Yama Pills.

关 键 词：高效液相色谱法 亚玛丸 羟基红花黄色素A 

分 类 号：R284.1[医药卫生—中药学]