维生素C对妊娠期精神束缚压力暴露SD大鼠子代认知功能的改善作用及其机制  

Improvement and mechanism of vitamin C diet on cognitive function of offspring of SD rats exposed to mental restraint stress during pregnancy

作　　者：程艳 郭建伟 赵静[1] 李扬[1] CHENG Yan;GUO Jianwei;ZHAO Jing;LI Yang(Department of Anesthesia,Shaanxi Provincial People’s Hospital,Xi'an 710068,China)

机构地区：[1]陕西省人民医院麻醉科,西安710068

出　　处：《山东医药》2023年第15期22-27,共6页Shandong Medical Journal

基　　金：陕西省自然科学基础研究计划(2023-JC-QN-0961)。

摘　　要：目的明确精神束缚压力暴露SD大鼠妊娠期饮食中加入维生素C(VitC)是否能改善子代鼠认知功能,并了解其作用机制。方法将63只健康清洁SD大鼠(雄鼠14只,雌鼠49只)分为VitC1组、VitC2组、VitC3组、VitC4组、VitC5组、压力暴露组(PE组)、对照组(C组),每组7只雌性和2只雄性SD大鼠,将2~3月龄雌性与雄性SD大鼠(3∶1)放置过夜进行交配,次日晨检查雌性SD大鼠阴道涂片,确定涂片为阳性的日期即为胚胎第0天。VitC1组、VitC2组、VitC3组、VitC4组、VitC5组妊娠SD大鼠胚胎第0天饮食中加入3.1、10.4、31.2、62.4、104.4 mg/(kg·d)VitC,在妊娠第14~20天每天3次暴露于精神束缚压力下,持续45 min~1 h;PE组:压力暴露同上,饮食中未加入VitC。对照组:无压力束缚暴露,饮食中未加入VitC。对1月龄子代鼠进行Morris水迷宫和Y迷宫测试。上述行为学测试完成后,断头处死子代鼠,取脑组织,测定脑细胞中NADH-CoQ氧化还原酶(线粒体复合物Ⅰ)、琥珀酸-CoQ氧化还原酶(线粒体复合物Ⅱ),脑组织中SOD、MDA、ROS及GCL活性。结果Morris水迷宫定位航行试验中,压力暴露第3、4天,与C组子代鼠相比,PE组潜伏期延长(P<0.05);与PE组相比,VitC2组及VitC3组潜伏期缩短(P均<0.05)。在Morris水迷宫空间探索试验中,与C组子代鼠相比,PE组子代鼠平台所在象限滞留时间百分比和穿越平台有效区域次数减少(P均<0.05);与PE组相比,VitC2组与VitC3组子代鼠平台所在象限滞留时间百分比和穿越平台有效区域次数增加(P均<0.05)。与C组子代鼠相比,PE组子代鼠Y迷宫新颖臂探索距离、探索次数及探索时间增加(P均<0.05);与PE组相比,VitC2组与VitC3组子代鼠Y迷宫新颖臂探索距离、探索次数及探索时间减少(P均<0.05)。与C组相比,PE组子代鼠脑细胞中线粒体复合物Ⅰ和复合物Ⅱ活性下降(P均<0.05);与PE组相比,VitC1、VitC2及VitC3组子代鼠脑细胞中线粒体复合物Ⅰ和复合物Ⅱ活性增加(Objective To investigate whether vitamin C dietary supplementation improves cognitive function of off⁃spring of SD rats exposed to mental restraint stress during pregnancy.Methods Sixty-three healthy clean SD rats(14 males,49 females)were divided into groups VitC1,VitC2,VitC3,VitC4,and VitC5,pressure exposure group(group PE),and control group(group C),with 7 female and 2 male SD rats in each group;2-3 months old female SD rats and male SD rats(3:1)were placed overnight for mating.The vaginal smears of the female SD rats were checked on the next morning;when the smear was positive,we took the time as day 0 of the embryo.VitC of 3.1,10.4,31.2,62.4,and 104.4 mg/(kg d)were added to the diet of pregnant SD rat in the groups VitC1,VitC2,VitC3,VitC4,and VitC5 from day 0 of pregnancy,and pregnant SD rat were exposed to mental restraint pressure 3 times a day,for 45 min-1 h;in the group PE,we did the same stress exposure as above without addition of VitC to diet;in the group C,rats were not ex⁃posed to mental restraint pressure,and no VitC was added to the diet.Behavioral tests(Morris water maze and Y maze tests)were performed on 1-month-old offspring.After the behavioral tests,the offspring mice were executed with decapita⁃tions and the activities of NADH-CoQ oxidoreductase(complex I),succinate-CoQ oxidoreductase(complex II),superox⁃ide dismutase(SOD),malondialdehyde(MDA),reactive oxygen species(ROS),and glutamate cysteine ligase(GCL)in the brain tissue homogenates were measured.Results In the Morris water maze navigation test,on the 3rd and 4th days of pressure exposure,compared with group C,the average latency in offspring of group PE was prolonged(P<0.05);com⁃pared with the group PE,the average latency in offspring of groups VitC2 and VitC3 were shortened(P<0.05).In the Mor⁃ris water maze space exploration test,compared with group C,the percentage of the residence time in the quadrant of the platform and the frequency of crossing the effective area in offspring decreased in the group PE(all P<0.05);compare

关 键 词：维生素C 认知功能 动物模型 妊娠期精神束缚压力暴露动物模型 线粒体损伤 氧化应激 

分 类 号：R3[医药卫生—基础医学]