Myogenesis controlled by a long non-coding RNA 1700113A16RIK and post-transcriptional regulation  

作　　者：Xin Fu Sheng Li Minzhi Jia Bo Xu Lele Yang Ruimiao Ma Hong Cheng Wenjun Yang Ping Hu 

机构地区：[1]Spine Center,Department of Pediatric Orthopedics,Xin Hua Hospital Affiliated to Shanghai Jiao Tong University,School of Medicine,Shanghai 200092,China [2]State Key Laboratory of Cell Biology,Shanghai Institute of Biochemistry and Cell Biology,Center for Excellence in Molecular Cell Science,Chinese Academy of Sciences,Shanghai 200031,China [3]Guangzhou Laboratory,Guangzhou 510700,Guangdong,China [4]State Key Laboratory of Molecular Biology,Shanghai Institute of Biochemistry and Cell Biology,Center for Excellence in Molecular Cell Science,Chinese Academy of Sciences,Shanghai 200031,China [5]Institute for Stem Cell and Regeneration,Chinese Academy of Sciences,Beijing 100101,China

出　　处：《Cell Regeneration》2022年第1期120-130,共11页细胞再生（英文）

基　　金：This work was supported by the Strategic Priority Research Program of the Chinese Academy of Science(XDA16020400 to P.H.);Ministry of Science and Technology of China(2017YFA0102700 to P.H.);the National Natural Science Foundation of China(32170804 to P.H.and 81200355 to W.Y.);CAS-Youth Innovation Program Association(2016246 to W.Y.);Shanghai Natural Science Foundation(18ZR1446300 to W.Y.).

摘　　要：Long non-coding(lnc)RNA plays important roles in many cellular processes.The function of the vast majority of lncRNAs remains unknown.Here we identified that lncRNA-1700113A16RIK existed in skeletal muscle stem cells(MuSCs)and was significantly elevated during MuSC differentiation.Knockdown of 1700113A16RIK inhibits the differentiation of muscle stem cells.In contrast,overexpression of 1700113A16RIK promotes the differentiation of muscle stem cells.Further study shows the muscle specific transcription factor Myogenin(MyoG)positively regulates the expression of 1700113A16RIK by binding to the promoter region of 1700113A16RIK.Mechanistically,1700113A16RIK may regulate the expression of myogenic genes by directly binding to 3’UTR of an important myogenic transcription factor MEF2D,which in turn promotes the translation of MEF2D.Taken together,our results defined 1700113A16RIK as a positive regulator of MuSC differentiation and elucidated a mechanism as to how 1700113A16RIK regulated MuSC differentiation.

关 键 词：Long non-coding RNA(lncRNA) 1700113A16RIK Muscle stem cell(MuSC)differentiation Myocyte-specific enhancer binding factor 2(MEF2D) 

分 类 号：Q2[生物学—细胞生物学] Q78