Beclin1 haploinsufficiency compromises mesenchymal stem cell-offered cardioprotection against myocardial infarction  

作　　者：Xing Qin Juanjuan Fei Yu Duan Asli F.Ceylan Fuyang Zhang Jun Ren 

机构地区：[1]Department of Cardiology,Xijing Hospital,Fourth Military Medical University,Xi’an 710032,Shaanxi,China [2]Department of Cardiology and Shanghai Institute of Cardiovascular Diseases,Zhongshan Hospital Fudan University,Shanghai 200032,China [3]Department of Medical Pharmacology,Ankara Yildirim Beyazit University,Faculty of Medicine,Bilkent,Ankara,Turkey [4]Department of Laboratory Medicine and Pathology,University of Washington,Seattle,WA 98195,USA

出　　处：《Cell Regeneration》2022年第1期192-202,共11页细胞再生（英文）

基　　金：This work was supported in part by a grant from Natural Science Foundation of China(91749128).

摘　　要：Mesenchymal stem cells(MSCs)-based therapy has displayed some promises in ischemia heart diseases although its efficacy may be affected by changes in surrounding environments.This study evaluated the role of autophagy insuf-ficiency using Beclin1 haploinsufficiency(BECN^(+/−))on intra-myocardial MSC transplantation-evoked effect against myocardial infarction.Donor MSCs from C57BL/6 mice were labelled with cell-tracker CM Dil and were delivered into LV free wall adjacent to infarct region in wild-type(WT)and BECN^(+/−) recipient mice following ligation of left main coronary artery(MI-MSCs).Ten days following MI,myocardial function was assessed using echocardiography.Cardiomyocyte contractility and intracellular Ca^(2+) were monitored using cardiomyocytes from the area-at-risk adjacent to infarct.CM-Dil labeled cells were tracked in MSCs recipient mice using fluorescence microscopy.Lectin,Masson trichrome staining and Western blot analysis were employed to determine cardiomyocyte area,scar fibrosis,apoptosis and inflammation.MI insult triggered scar fibrosis,LV chamber dilation,decreased fractional shortening,ejection fraction,cardiomyocyte shortening,maximal velocity of shortening and relengthening as well as prolonged relength-ening,which were abrogated or attenuated by MSCs therapy in WT but not BECN^(+/−)mice.MI decreased intracellular Ca^(2+) rise and decay in response to electrical stimuli without affecting resting intracellular Ca^(2+),which were reconciled by MSCs in WT but not BECN^(+/−) mice.MSCs further attenuated MI-induced mitochondrial ultrastructural injury,apoptosis,inflammation and autophagy defects in periinfarct area in WT but not BECN^(+/−)mice.Collectively,our results suggested that autophagy insufficiency dampened in MSCs-elicited cardioprotection associated with dampened apoptosis and inflammation.

关 键 词：Myocardial infarction MSCs BECLIN1 CONTRACTION Apoptosis Autophagy 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]