A mouse model of vitiligo based on endogenous auto-reactive CD8+T cell targeting skin melanocyte  

作　　者：Daoming Chen Zijian Xu Jun Cui Ting Chen 

机构地区：[1]National Institute of Biological Sciences,Beijing,China [2]Tsinghua Institute of Multidisciplinary Biomedical Research,Tsinghua University,Beijing,China

出　　处：《Cell Regeneration》2022年第1期316-332,共17页细胞再生（英文）

基　　金：This work was supported by grants from the National Key R&D Program of China(2017YFA0103500,2021YFA1101000).

摘　　要：Vitiligo is the most common human skin depigmenting disorder.It is mediated by endogenous autoreactive CD8+T cells that destruct skin melanocytes.This disease has an estimated prevalence of 1%of the global population and cur-rently has no cure.Animal models are indispensable tools for understanding vitiligo pathogenesis and for developing new therapies.Here,we describe a vitiligo mouse model which recapitulates key clinical features of vitiligo,including epidermis depigmentation,CD8+T cell infiltration in skin,and melanocyte loss.To activate endogenous autoreactive cytotoxic CD8+T cells targeting melanocytes,this model relies on transient inoculation of B16F10 melanoma cells and depletion of CD4+regulatory T cells.At cellular level,epidermal CD8+T cell infiltration and melanocyte loss start as early as Day 19 after treatment.Visually apparent epidermis depigmentation occurs 2 months later.This protocol can efficiently induce vitiligo in any C57BL/6 background mouse strain,using only commercially available reagents.This enables researchers to carry out in-depth in vivo vitiligo studies utilizing mouse genetics tools,and provides a powerful platform for drug discovery.

关 键 词：VITILIGO Mouse model Activation Endogenous autoreactive cytotoxic CD8+T cells 

分 类 号：R392[医药卫生—免疫学]