超高效液相色谱法同时测定红芪和黄芪药材中有效成分含量  

Simultaneous Determination of Effective Components in Hedysari Radix and Astragali Radix by UPLC

作　　者：焦洁 郭建博 郑旭 胡萌萌 蔡虎 JIAO Jie;GUO Jianbo;ZHENG Xu;HU Mengmeng;CAI Hu(Shaanxi Institute for Food and Drug Control·NMPA Key Laboratory of Drug Microbiological Detection Technology,Xi'an,Shaanxi,China 710065)

机构地区：[1]陕西省食品药品检验研究院·国家药品监督管理局药品微生物检测技术重点实验室,陕西西安710065

出　　处：《中国药业》2023年第12期84-88,共5页China Pharmaceuticals

基　　金：陕西省重点研发计划[2019SF-075];陕西省“医研校企”中医药传承创新平台[陕中医药函(2022)54号-5]。

摘　　要：目的建立同时测定红芪和黄芪药材中有效成分含量的超高效液相色谱法。方法色谱柱为Waters BEH C18柱(200 mm×2.1 mm,1.7μm),流动相为乙腈-0.1%甲酸水溶液(梯度洗脱),流速为0.2 mL/min,检测波长为250 nm,柱温为35℃,进样量为1μL。结果香草酸、阿魏酸、毛蕊异黄酮苷、异阿魏酸、芒柄花苷、毛蕊异黄酮质量浓度分别在6.05~121.00μg/mL、4.37~87.32μg/mL、0.30~6.02μg/mL、1.09~21.81μg/mL、6.10~122.12μg/mL、1.00~20.05μg/mL范围内与峰面积线性关系良好(R^(2)≥0.9996);精密度、稳定性、重复性试验结果的RSD均小于5.0%;平均加样回收率分别为98.26%,96.72%,98.78%,97.56%,99.18%,99.19%,RSD均小于4.0%(n=6)。武都红芪药材样品和蒙古黄芪药材样品的共有成分毛蕊异黄酮苷、芒柄花苷、毛蕊异黄酮含量差异较大,分别为2.73,46.61,8.45μg/g和42.36,13.03,37.45μg/g。结论所建立的方法可靠,操作简单,可为后期红芪及黄芪药材的质量控制提供参考。Objective To establish an ultra-high-performance liquid chromatography(UPLC)method for the simultaneous determination of effective components in Hedysari Radix and Astragali Radix.Methods The chromatographic column was Waters BEH C18 column(200 mm×2.1 mm,1.7μm),the mobile phase was acetonitrile-0.1%formic acid aqueous solution(gradient elution),the flow rate was 0.2 mL/min,the detection wavelength was 250 nm,the column temperature was 35℃,and the injection volume was 1μL.Results The linear ranges of vanillic acid,ferulic acid,calycosin-7-glucoside,isoferulic acid,ononin and calycosin were 6.05-121.00μg/mL,4.37-87.32μg/mL,0.30-6.02μg/mL,1.09-21.81μg/mL,6.10-122.12μg/mL and 1.00-20.05μg/mL respectively(R^(2)≥0.9996).The RSDs of precision,stability and repeatability tests were all lower than 5.0%.The average recovery rates of the above six components were 98.26%,96.72%,98.78%,97.56%,99.18%and 99.19%respectively,with RSDs lower than 4.0%(n=6).The contents of calycosin-7-glucoside,ononin and calycosin in Hedysari Radix samples(Wudu)were 2.73,46.61 and 8.45μg/g respectively,which were significantly different from 42.36,13.03 and 37.45μg/g in Astragali Radix samples(Inner Mongolia)respectively.Conclusion The established method is reliable and simple,which can provide a reference for the quality control of Hedysari Radix and Astragali Radix in the later stage.

关 键 词：红芪 黄芪 超高效液相色谱法 共有成分 含量测定 毛蕊异黄酮苷 芒柄花苷 毛蕊异黄酮 

分 类 号：R917[医药卫生—药物分析学] R927[医药卫生—药学]