基于MEMS技术的高纯度外泌体磁分离微流控芯片  

Microfluidic Chips Based on MEMS Technology for High-Purity Exosome Magnetic Separation

作　　者：王菲 林树靖[1,2] 郅晓 吁子贤 陈迪 Wang Fei;Lin Shujing;Zhi Xiao;Yu Zixian;Chen Di(Department of Instrument Science and Engineering,School of Electronic Information and Electrical Engineering,Shanghai Jiao Tong University,Shanghai 200240,China;Shanghai Engineering Research Center for Intelligent Diagnosis and Treatment Instrument,Shanghai 200240,China;Institute for Personalized Medicine,School of Biomedical Engineering,Shanghai Jiao Tong University,Shanghai 200030,China)

机构地区：[1]上海交通大学电子信息与电气工程学院仪器科学与工程系,上海200240 [2]上海智能诊疗仪器工程技术研究中心,上海200240 [3]上海交通大学生物医学工程学院个性化医学研究院,上海200030

出　　处：《微纳电子技术》2023年第5期729-736,共8页Micronanoelectronic Technology

基　　金：国家自然科学基金青年科学基金项目(82003274);上海市科委项目(21S31906400,21ZR1435000);上海交通大学医工交叉项目(YG2022QN083)。

摘　　要：基于CD63核酸适配体功能化磁珠捕获外泌体,设计和制作了一种可从胃癌细胞上清液中分离出高纯度外泌体的微流控芯片,芯片内磁增强微结构的梳齿间距40μm,最小宽度仅为20μm。利用COMSOL软件对磁增强微结构进行磁场仿真,结果表明最大磁感应强度和磁场梯度分别可达15.1 T和5.77×10^(4)T/m。通过纳米粒度Zeta电位仪对链霉亲和素磁珠与CD63核酸适配体偶联形成的功能化磁珠(SAMNP@Apt)进行表征,结果表明它们实现了良好偶联。使用SAMNP@Apt捕获外泌体,捕获效率可达92.7%。使用微电子机械系统(MEMS)技术制备了磁分离微流控芯片,并在微流控芯片中进行了外泌体磁分离实验,结果表明微流控芯片的外泌体分离纯度可达88.75%。Based on CD63-aptamer functionalized superparamagnetic beads,a microfluidic chip was designed and fabricated to separate high purity exosomes from gastric cancer cell supernatant.The magnetically enhanced microstructure in the chip has a comb pitch of 40μm and a minimum width of only 20μm.The magnetic field of the magnetic enhanced microstructure was simulated using COMSOL software.The results show that the maximum magnetic induction intensity and magnetic field gradient can reach 15.1 T and 5.77×10^(4) T/m,respectively.The functionalized superparamagnetic beads(SAMNPs@Apt)were modified with CD63-aptamer by specific reaction between streptavidin on the surface of beads with biotin on the end of CD63-aptamers.The characterization result of the SAMNPs@Apt by nanoparticle Zeta potential analyzer shows that they achieve good coupling.The SAMNPs@Apt was used to capture exosomes with a capture efficiency of 92.7%.The magnetic separation microfluidic chip was fabricated by micro-electromechanical system(MEMS)technology,and the magnetic separation experiment of exosomes was performed in the microfluidic chip.The results show that the purity of exosome separation in the microfluidic chip can reach 88.75%.

关 键 词：微电子机械系统(MEMS) 微流控芯片 外泌体磁分离 核酸适配体 磁增强微结构 

分 类 号：TH703[机械工程—仪器科学与技术] O35[机械工程—精密仪器及机械] Q503[理学—流体力学]