Analysis of codon usage patterns in Bupleurum falcatum chloroplast genome  

作　　者：Mengqi Gao Xiaowei Huo Liting Lu Mengmeng Liu Gang Zhang 

机构地区：[1]College of Traditional Chinese Medicine,Hebei University,Baoding 071002,China [2]College of Pharmaceutical Science,Institute of Life Science and Green Development,Hebei University,Baoding 071002,China [3]College of Pharmacy and Shaanxi Qinling Application Development and Engineering Center of Chinese Herbal Medicine,Shaanxi University of Chinese Medicine,Xi’an 712046,China

出　　处：《Chinese Herbal Medicines》2023年第2期284-290,共7页中草药（英文版）

基　　金：supported by the National Natural Science Foundation of China(82204567,81973430);Natural Science Foundation of Hebei Province(H2020201006);Medical Science Foundation of Hebei University(2021B10);China University Student Innovation and Entrepreneurship Training Program Project(2022333);Advanced Talents Incubation Program of the Hebei University(521000981177,521000981170);Resources Development of“Qin Drug”of Shaanxi University of Chinese Medicine Innovation Team Project(2019-QN01).

摘　　要：Objective In order to distinguish the traditional Chinese medicine Bupleurum falcatum and its adulterants effectively and develop a better understanding of the factors affecting synonymous codon usage,codon usage patterns of chloroplast genome,we determine the complete chloroplast(cp)genome of B.falcatum and clarify the main factors that influence codon usage patterns of 78 genes in B.falcatum chloroplast genome.Methods The total genomic DNA of fresh leaves from a single individual of B.falcatum was extracted with EASYspin plus Total DNA Isolation Kit and 2μg genome DNA was sequenced using Illumina Hiseq 2500 Sequencing Platform.The cp genome of B.falcatum was reconstructed with MITObim v1.8 and annotated in the program CPGAVAS2 with default parameters.Python script and Codon W were used to calculate the codon usage bias parameters.Results The full length of B.falcatum cp genome was 155851 bp,132 different genes were annotated in this cp genome containing 80 protein-coding genes,30 tRNA genes,and four rRNA genes.The codon usage models tended to use A/T-ending codons.The neutrality plot,ENC plot,PR2-Bias plot and correspondence analysis showed that both compositional constraint under selection and mutation could affect the codon usage models in B.falcatum cp genome.Furthermore,three optimal codons were identified and most of these three optimal codons ended with G/U.Conclusion The cp genome of B.falcatum has been characterized and the codon usage bias in B.falcatum cp genome is influenced by natural selection,mutation pressure and nucleotide composition.The results will provide much more barcode information for species discrimination and lay a foundation for future research on codon optimization of exogenous genes,genetic engineering and molecular evolution in B.falcatum.

关 键 词：Bupleurum falcatum L. chloroplast genome codon usage 

分 类 号：R282.5[医药卫生—中药学]