Cytotoxic sesquiterpene aryl esters from Armillaria gallica 012m  

作　　者：Yanping Li Shuizhu Lou Run Yang Ling Zhang Qiuping Zou Shanzhai Shang Lu Gao Weiguang Wang 

机构地区：[1]School of Chinese Pharmacy,Yunnan University of Chinese Medicine,Kunming 650500,China [2]Key Laboratory of Natural Products Synthetic Biology of Ethnic Medicinal Endophytes,State Ethnic Affairs Com-mission,Key Laboratory of Chemistry in Ethnic Medicinal Resources,Ministry of Education,Yunnan Minzu University,Kunming 650031,China [3]Key Laboratory of Tobacco Chemistry of Yunnan Province,China Tobacco Yunnan Industrial Co.,Ltd.,Kunming 650231,China [4]Hongta Tobacco(Group)Co.,Ltd.,Yuxi 653100,China

出　　处：《Chinese Herbal Medicines》2023年第2期343-346,共4页中草药（英文版）

基　　金：This project was in part supported financially by National Natural Science Foundation of China(Nos.31960095 and 82160670);National Natural Science Foundation of Yunnan Province(Nos.202101AS070022 and 202201AT070228);Yunnan Ten thousand Talents Program to Weiguang Wang and Yanping Li.

摘　　要：Objective:To study the chemical constituents of the EtOAc extract of Armillaria gallica 012m.Methods:The chemical constituents of the EtOAc extract of A.gallica 012m were isolated and purified by various column chromatography and their structures were elucidated on the basis of the 1D and 2D NMR spectroscopic and HRESIMS data.Cytotoxicity of all isolates against A549,HCT-116,M231 and W256 human tumor cells was determined by the MTT method.Results:A new sesquiterpene aryl ester,armimelleolide C(1),and eight known ones including armillarivin(2),melleolide F(3),6'-chloromelleolide F(4),melleolide(5),melleolide K(6),melledonol(7),13-hydroxydihydromelleolide(8),and armillane(9),were isolated from the EtOAc extract of A.gallica 012m.All isolates showed potential cytotoxic activities against at least one of the human cancer cell lines with IC_(50) values ranging from(3.17±0.54)to(17.57±0.47)μmol/L.Compound 1 showed significant inhibitory activity against M231 with an IC_(50) value of(7.54±0.24)μmol/L compared with paclitaxel as the positive control.Compounds 2,3,and 7,9 showed obvious inhibitory activity against HCT-116 and were better than that of the positive control.Conclusion:The chemical constituents including a new sesquiterpene aryl ester armimelleolide C(1)from the EtOAc extract of A.gallica 012m have a variety of structures and potential antitumor activities.

关 键 词：Armillaria gallica 012m armimelleolide C cytotoxic activity sesquiterpene aryl esters TRICHOLOMATACEAE 

分 类 号：R285[医药卫生—中药学]