不同土壤氮水平下C_(3)和C_(4)植物之间的菌根网络及氮传递  

Interplant transfer of nitrogen between C_(3) and C_(4) plants through common mycorrhizal networks under different nitrogen availability

作　　者：Muhammad Atif Muneer Xiaohui Chen Muhammad Zeeshan Munir Zaib-Un Nisa Muhammad Abu Bakar Saddique Shehzad Mehmood Da Su Chaoyuan Zheng Baoming Ji 

机构地区：[1]College of Grassland Science,Beijing Forestry University,Beijing 100083,China [2]College of Resources and Environment/International Magnesium Institute,Fujian Agriculture and Forestry University,Fuzhou 350002,China [3]Research Centre of Phosphorous Efficient Utilization and Water Environment Protection along the Yangtze River Economic Belt,College of Resources and Environment,Anhui Agricultural University,Hefei 230036,China [4]School of Environment and Energy,Peking University Shenzhen Graduate School,Shenzhen 518055,China [5]College of Biological Science and Technology,Beijing Forestry University,Beijing 100083,China [6]Cotton Research Institute,Multan,Punjab,Pakistan [7]Institute of Plant Breeding and Biotechnology,Muhammad Nawaz Shareef University of Agriculture,Multan,Pakistan [8]Department of Plant Sciences,Quaid-i-Azam University,Islamabad 45320,Pakistan [9]Department of Environmental Sciences,COMSATS University Islamabad,Vehari Campus,Vehari 61100,Pakistan [10]Key Laboratory of Ministry of Education for Genetics,Breeding and Multiple Utilization of Crops,College of Agriculture,Fujian Agriculture and Forestry University,Fuzhou 350002,China

出　　处：《Journal of Plant Ecology》2023年第2期28-38,共11页植物生态学报（英文版）

基　　金：Foundation of China(31761123001-1,31770542)financially supported this work。

摘　　要：丛枝菌根真菌(AMF)的菌丝在土壤中通常可以链接相同或不同物种的植物根系,所形成的菌根网络可以将养分从一种植物传递到另一种植物。然而,土壤中的氮(N)含量对不同植物物种之间通过菌根网络进行养分转移的影响尚未实验验证。为了定量研究不同土壤氮水平下羊草(LC)和糙隐子草(CS)之间通过菌根网络的养分转移,本研究建立了两个分室盆栽系统,即CS–LC系统(CS和LC分别为供体和受体)和LC–CS系统(LC和CS分别为供体和受体),设置了3种土壤氮添加水平,即不添加氮对照(N0)、添加7 mg/kg(N1)和添加14 mg/kg(N2)。通过在供体植物一侧土壤添加稳定同位素^(15)N,示踪并量化不同物种植物幼苗之间通过菌根网络传递的氮。在CS–LC系统中,AMF侵染率、菌丝密度和植物生物量在N1水平最高;而在LC–CS系统,AMF侵染率、菌丝密度和植物生物量在N2水平达到最大。此外,在CS–LC系统中,^(15)N转移率介于16%到61%之间,在N1水平最大。对于LC–CS系统,^(15)N传输速率相对较低,最大值出现在N0水平。这些发现表明,土壤中的氮含量能够调控供体植物和受体植物之间经由菌根网络传递的氮养分。Hyphae of arbuscular mycorrhizal fungi(AMF)in soil often form complex mycorrhizal networks among roots of same or different plant species for transfer of nutrients from one plant to another.However,the effect of soil nitrogen(N)availability on nutrient transfer between different plant species via common mycorrhizal networks(CMNs)has not been experimentally examined.In order to quantify CMN-mediated nutrient transfer between Leymus chinensis(LC)and Cleistogene squarrosa(CS),two systems,i.e.the CS–LC system(CS and LC were donor and recipient,respectively)and the LC–CS system(LC and CS were donor and recipient,respectively)were established.Stable isotopic ^(15)N was applied to track N transfer between heterospecific seedlings connected by CMNs under three levels of soil N additions:no N addition control(N0),N addition with 7 mg/kg(N1)and N addition with 14 mg/kg(N2).In the CS–LC system,the highest rate of AMF colonization and hyphal length density(HLD)were found at N1.In contrast,maximum AMF colonization rate and HLD were recorded at N2 in LC–CS system.Consequently,plant biomass was significantly higher under N1 and N2 levels in CS–LC and LC–CS systems,respectively.Moreover,in CS–LC system,^(15)N transfer rate ranged from 16%to 61%,with maximum transfer rate at N1.For LC–CS system,^(15)N transfer rate was much lower,with the maximum occurring at N0.These findings suggest that CMNs could potentially regulate N transfer from a donor to recipient plant depending upon the strength of individual plant carbon sink.

关 键 词：菌根网络 氮传递 差异响应 糙隐子草(Cleistogene squarrosa) 羊草(Leymus chinensis) 典型草原 

分 类 号：Q94[生物学—植物学]