工业大麻叶片内生细菌分离鉴定及系统发育分析  

Isolation,Identification and Phylogenetic Analysis of Endophytic Bacteria in Leaves of Industrial Hemp(Cannabis sativa)

作　　者：马军[1] 汪洪敏 陈瑛[2,4] 苑泽宁 张明[1] 房郁妍[1] 胡莹莹[1] 唐立郦[1] MA Jun;WANG Hongmin;CHEN Ying;YUAN Zening;ZHANG Ming;FANG Yuyan;HU Yingying;TANG Lili(Institute of Industrial Crops,Heilongjiang Academy of Agricultural Sciences,Harbin 150086;College of Life Science and Technology,Harbin Normal University,Harbin 150086;School of Marine Life,Ocean University of China,Qingdao 26600;Harbin Institute of Technology(Shenzhen),Shenzhen 518000,China)

机构地区：[1]黑龙江省农业科学院经济作物研究所,哈尔滨150086 [2]哈尔滨师范大学生命科学与技术学院,哈尔滨150086 [3]中国海洋大学海洋生命学院,山东青岛266000 [4]哈尔滨工业大学(深圳校区),广东深圳518000

出　　处：《东北农业科学》2023年第2期55-59,共5页Journal of Northeast Agricultural Sciences

基　　金：黑龙江省政府博士后资助项目(LBH-Z17204);黑龙江省农业科学院科研基金项目(2018YYYF033,2019YYYF010)。

摘　　要：植物内生菌是重要的微生物资源,筛选分离植物内生菌对挖掘和利用有益微生物有重要作用。为初步了解工业大麻内生细菌生物多样性,本研究采用微生物学传统分离培养方法从黑龙江省工业大麻果期叶中分离出的6株菌株进行形态学观察、革兰氏染色观察,通过16S rDNA序列进行分子鉴定,并通过BLAST软件同源序列比对构建进化树进行系统发育分析。结果表明:在获得成功测序的6株菌株中,其中5株为革兰氏阳性菌,1株为革兰氏阴性菌,其形态常见为杆状,极个别为球状。16S rDNA测序结果采用BLAST软件同源性比对分析,表明菌株DM02、DM03、DM04、DM05和DM06为Bacillus属,相似性99.85%~100%;DM01菌株为Acinetobacter属,最高相似性99.93%。通过构建系统发育树,可以明显地看出这6株内生细菌被划分为2大支。本研究为开发和利用工业大麻内生有益细菌提供理论参考。Phytoendophytes are important microbial resources.Isolation and identification of phytoendophytes provide an important base for the utilization of beneficial plant microbes.In order to preliminarily judge the phylogenetic relationship of endophytic bacteria in industrial hemp and further understand its biodiversity,using the traditional isolation and culture method,we isolated six bacteria strains from the leaves of an industrial hemp variety from Heilongjiang Province.The morphological,Gram coloring,16s rDNA sequences and phylogenetic analysis were carried out.The morphology of the six strains were spherical.Five of them were Gram-positive bacteria,and one was Gram-negative bacterium.16S rDNA BLAST result indicated that five strains were Bacillus,with 99.85%-100%similarity;and the other one was Acinetobacter,with 99.93%maximum similarity.By constructing phylogenetic tree,the six endophytic bacteria were obviously aggregated into two branches.The result can provide references for the exploitation and utilization of endophitic bacteria in Cannabis sativa.

关 键 词：工业大麻 内生细菌 分离鉴定 系统发育分析 

分 类 号：S563.3[农业科学—作物学]