酉州乌羊五个组织的权重基因共表达网络分析  

Weighted Gene Co-expression Network Analysis of Five Tissues in Youzhou Dark Goat(Capra hircus)

作　　者：陈灿灿 蒋婧[1,2] 孙晓燕 李杰[1,2] 刘良佳 王高富[1,2] 周鹏 任航行[1,2] CHEN Can-Can;JIANG Jing;SUN Xiao-Yan;LI Jie;LIU Liang-Jia;WANG Gao-Fu;ZHOU Peng;REN Hang-Xing(Chongqing Academy of Animal Sciences,Chongqing 402460,China;Chongqing Center of Goat Engineering Technology Research,Chongqing 402460,China)

机构地区：[1]重庆市畜牧科学院,重庆402460 [2]重庆市山羊工程技术研究中心,重庆402460

出　　处：《农业生物技术学报》2023年第7期1450-1463,共14页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(31672393);重庆市科研院所绩效激励引导专项(cstc2021jxj180018);重庆市级财政项目(22544C-22)。

摘　　要：酉州乌羊(Capra hircus)是地方特色山羊遗传资源,研究其特色性状形成机制具有重要意义。本研究收集酉州乌羊心脏、肝脏、背最长肌、前唇、皮肤(每个组织各3个样本)共计15个样本的转录组数据进行分析,采用权重基因共表达网络分析(weighted gene co-expression network analysis,WGCNA)软件包构建共表达网络,并对特异性模块中的基因进行GO和KEGG富集分析。结果显示,心脏和肝脏各鉴定出1个特异表达模块,其中心脏特异性蓝色模块内基因主要富集到柠檬酸循环信号通路和各种代谢过程,肝脏特异性黄色模块内基因主要富集到过氧化物酶、氨基酸代谢过程和化学致癌等功能;宝石绿模块内基因主要富集到蛋白质结合等功能,绿色模块内基因主要富集到磷脂酰肌醇结合等功能,棕色模块内基因主要富集到细胞蛋白质代谢等生物学过程。通过CytoHubba插件算法,将宝石绿、蓝色和棕色模块中连通性靠前的10个基因定义为核心基因;利用qRT-PCR方法验证3个模块内6个核心基因的组织表达,结果表明,ADCY9和ADCY6基因在心脏和背最长肌表达量较高,AKT2和MAPK3基因在前唇组织高表达,CAMK2G和CAMK2B基因在背最长肌高表达,与转录组测序结果基本一致。上述结果对于深入开展酉州乌羊功能基因的生物信息学研究具有参考价值。本研究为我国地方山羊遗传资源特色性状的挖掘及开发利用提供基础资料。Youzhou dark goat(Capra hircus)is the genetic resources of local characteristic goat.It is of great significance to study the forming mechanism of its characteristic traits.This study collected the transcriptome data of a total of 15 samples from the heart,liver,longissimus dorsi,anterior lip and skin of Youzhou dark goat(3 samples for each tissue)for analysis.The weighted gene co-expression network analysis(WGCNA)software package was used to construct the co-expression network,and the GO and KEGG enrichment analysis was performed on the genes in the specific modules.Results showed that one specific expression module was identified in the heart and liver respectively.The genes in the heart specific blue module were mainly enriched in the citric acid cycle signal pathway and various metabolic processes.The genes in the liver specific yellow module were mainly enriched in the functions of peroxidase,amino acid metabolic process and chemical carcinogenesis.Genes in the turquoise module are mainly enriched in protein binding and other functions,genes in the green module are mainly enriched in phosphatidylinositol binding and other functions,and genes in the brown module are mainly enriched in biological processes such as cell protein metabolism.Through the CytoHubba plug-in algorithm,the 10 genes with the highest connectivity in the turquoise,blue and brown modules were defined as core genes.The tissue expression level of 6 core genes in the 3 modules were verified by qRT-PCR,and the results showed that ADCY9 and ADCY6 genes had the highest expression in heart tissue,AKT2 and MAPK3 genes were highly expressed in the anterior lip tissue,and the CAMK2G and CAMK2B genes were highly expressed in the longissimus dorsi,which were consistent with the transcriptome sequencing results.The above results have reference value for further bioinformatics research on functional genes of Youzhou dark goat.This study provides basic data for the mining,development and utilization of characteristic traits of local goat genetic resource

关 键 词：酉州乌羊 权重基因共表达网络分析(WGCNA) 富集分析 核心基因 

分 类 号：S827[农业科学—畜牧学]