灰葡萄孢Homeodomain家族基因的鉴定与表达分析  

Identification and Expression Analysis of Homeodomain Family Genes in Botrytis cinerea

作　　者：李白 魏雅迪 刘英姿 曹宏哲 张康 邢继红 董金皋 LI Bai;WEI Ya-Di;LIU Ying-Zi;CAO Hong-Zhe;ZHANG Kang;XING Ji-Hong;DONG Jin-Gao(North China State Key Laboratry of Crop Improvement and Regulation,Jointly Established by the Province and the Ministry,Baoding 071000,China;Hebei Provincial Key Laboratory of Plant Physiology and Molecular Pathology,Baoding 071000,China;Landscape Management Center of Chengde City,Hebei Province,Chengde 067000,China)

机构地区：[1]省部共建华北作物改良与调控国家重点实验室,保定071000 [2]河北省植物生理与分子病理学重点实验室,保定071000 [3]河北省承德市园林管理中心,承德067000

出　　处：《农业生物技术学报》2023年第7期1513-1521,共9页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(31972217,32072369);中央引导地方科技发展基金(206Z6501G);河北省自然科学基金(C2022204040)。

摘　　要：Homeodomain家族基因在病原菌的分生孢子发育、致病过程以及胁迫响应过程中发挥重要作用。本研究利用生物信息学方法,在全基因组水平上对灰葡萄孢Homeodomain家族基因进行鉴定,并对其蛋白理化性质、系统进化关系、保守结构域及其在病菌分生孢子发育时期和侵染时期的表达规律进行分析;同时,利用qPCR技术,检测灰葡萄孢Homeodomain家族基因在NaCl、刚果红处理后的表达情况。结果发现,灰葡萄孢基因组中共包含14个Homeodomain家族基因,系统进化分析将其分为4个亚家族;灰葡萄孢Homeodomain家族基因均含有Homeodomain家族典型的SANT结构域,部分基因在分生孢子的不同发育时期和侵染时期上调表达,在NaCl和刚果红处理后Homeodomain家族基因表达水平存在明显差异,表明灰葡萄孢Homeodomain家族基因在病菌生长发育、侵染过程以及响应胁迫方面发挥重要的作用,本研究为阐明灰葡萄孢Homeodomain家族基因的功能及其作用机制提供了理论依据。Homeodomain transcription factors are widely present in plant pathogenic fungi and play an important role in the morphogenesis and infestation of pathogens.In this study,bioinformatics was used to identify the Homeodomain family genes of Botrytis cinerea at the genome-wide level.The physicochemical properties,phylogenetic relationships,conserved structural domains and expression patterns during the developmental and infestation periods of the pathogenic conidia of Homeodomain were carried out.The relative expression of Homeodomain treated with NaCl and Congo red were analysed by qPCR.The results showed that there were 14 Homeodomain family genes in the genome of Botrytis cinerea,which were classified into 4 subfamilies by phylogenetic analysis;All the Homeodomain family genes of Botrytis cinerea contained the typical SANT domain of the Homeodomain family,and some of them were up-regulated during different developmental and infestation periods of the conidia.The expression levels of the Homeodomain family genes were significantly different after NaCl and Congo red treatment,indicating that the Homeodomain family genes played an important role in the growth,development,the process of infestation,and the response to stress of the pathogen.The present study provides a theoretical basis for the elucidation of the functions of the Homeodomain family genes and their mechanisms of action.

关 键 词：灰葡萄孢 Homeodomain家族基因 生物信息学分析 表达分析 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]