猕猴桃蔗糖合酶基因家族鉴定及果实发育过程表达分析  

Identification of Sucrose Synthase Gene Family in Kiwifruit and Their Expression during Fruit Development

作　　者：陈成 王依 杨勇[1] 阎永齐[1] Chen Cheng;WangYi;YangYong;Yan Yongqi(Zhenjiang Institute of Agricultural Sciences in Hilly Area of Jiangsu Province,Jurong,212400;Yanliang Agricultural Technology Spreading Center,Xi'an,710089)

机构地区：[1]江苏丘陵地区镇江农业科学研究所,句容212400 [2]西安市阎良区农业技术推广中心,西安710089

出　　处：《分子植物育种》2023年第12期3866-3875,共10页Molecular Plant Breeding

基　　金：江苏现代农业产业技术体系建设专项(JATS[2022]449);镇江市科技创新资金项目(NY2021022)共同资助。

摘　　要：蔗糖合酶(SUS)被认为是库强的生物化学标志。为探明猕猴桃SUS基因家族特性及其在果实发育过程中与蔗糖代谢的作用,本研究运用生物信息学方法,对‘海沃德’(Hayward)猕猴桃SUS进行全基因组鉴定与系统发育关系、基因结构、蛋白质保守结构域、基序和染色体定位分析,利用实时荧光定量PCR技术分析果实发育过程中SUS家族基因的表达模式及与果实糖含量的关系。结果表明:猕猴桃中包含7个SUS基因;AdSUSs均含有保守的特征结构域:蔗糖合酶结构域和糖基转移酶结构域;SUS基因家族分为3个亚家族(SUSⅠ,SUSⅡ,SUSⅢ);位于同一亚家族的SUS具有相似的内含子/外显子分布模式及相似的蛋白质保守基序;AdSUS2.2与果实中蔗糖、果糖及葡萄糖含量相关性最高,说明其是调控猕猴桃果实蔗糖合成积累的重要基因。本研究结果可为猕猴桃AdSUSs基因功能解析及蔗糖代谢途径改造提供理论依据。Sucrose synthase(SUS)has been regarded as a biochemical marker for sink strength.In order to further explore the characteristics of SUS in kiwifruit and their role in sucrose metabolism during fruit development,the SUS gene families were identified from Hayward'kiwifruit genome database,and their phylogenetic relationship,gene structure,protein conserved domains,motifs and chromosomal locations were analyzed by bioinformatic approaches.On the other hand,quantitative real-time PCR was used to analyze the relationship between the expression patterns of SUS gene families and sugar concentrations during fruit development.The results showed that 7 SUS genes were identified in kiwifruit genome database,including 3 sbufamilies for SUS gene family(SUSⅠ,SUSⅡ,SUSⅢ).All AdsUSs had the sucrose synthase domain and glycosyl transferase domain.AdsUS genes in the same group exhibited similar intron/exon pattern and their coding proteins had similar conserved motifs.The correlation analysis showed that AdsUS2.2 had the highest correlation with sucrose,fructose and glucose content in kiwifruit.It suggests that AdSUS2.2 was the important gene in regulating the synthesis and accumulation of sucrose for kiwifruit.These results provide important reference for functional researches of AdSUSs and genetic modification of sucrose metabolism pathway in kiwifruit.

关 键 词：猕猴桃 蔗糖合酶 基因家族 表达特性 

分 类 号：S663.4[农业科学—果树学]